摘要
目的:建立温莪术的双向电泳技术(2-DE)体系。方法:采用TCA/丙酮沉降法提取蛋白,并用超速离心去除杂质。采用固相pH胶条在IPGphor TM Ⅲ等电聚焦系统上进行等电聚焦,采用Bio-Rad电泳仪进行SDS-PAGE电泳,以银染法染色。结果:得到了上百个点的双向电泳图谱。结论:本研究成功建立了温莪术蛋白双向电泳技术体系。
Objective： To establish the system of 2-D gel electrophoresis proteome of the leaf of Curcuma wenyujin. Methods： The total proteins of root were extracted with trichlo- roacetic acid （TCA）/acetone precipitation method and ultracentrifugation,and then separated by two-dimensional electrophoresis （2-DE）, which was an IPGphor TM unit with immobile pH gradient strips used in and the Ettan TM Dalt H system in SDS-PAGE. The 2-D gel was stained with silver. Results： Near hundred 2-D electrophorogram was gained by this method. Conclusion： A system of two-dimensional eleatronhoresis of curcuma wenvu.iin is successfully established.
出处
《温州医学院学报》
CAS
2009年第4期305-308,共4页
Journal of Wenzhou Medical College
基金
浙江省重大科技攻关项目(2005C13019)
温州市重大科技计划资助项目(S2005B001)
关键词
蛋白提取
双向电泳
TCA/丙酮沉降法
超速离心
protein extraction
two-dimensional electrophoresis（2-DE）
trichloroacetic acid （TCA）/acetone precipitation method
ultracentrifugation
