摘要
为了评估酵母表达的PCV2 Cap蛋白在猪体的免疫特性以及猪α干扰素对其的免疫佐剂效果,将酵母表达的PCV2 Cap蛋白配比适量的铝胶或重组猪α干扰素制成亚单位疫苗。30日龄仔猪分别接种铝胶佐剂亚单位疫苗和猪α干扰素佐剂亚单位疫苗,同时设攻毒对照与空白对照,首免后21 d加强免疫,二免后14 d用PCV2 JS株攻毒。铝胶佐剂亚单位疫苗免疫猪体后产生了PCV2特异性中和抗体。猪α干扰素佐剂亚单位疫苗组仔猪免疫后产生的ELISA抗体和中和抗体都显著高于铝胶亚单位疫苗组仔猪。攻毒对照组的4只仔猪攻毒后全都产生了病毒血症,并有较长时间的发热;铝胶亚单位疫苗组4头仔猪中只有1头仔猪产生病毒血症,添加猪α干扰素亚单位疫苗组的仔猪攻毒后都没有产生病毒血症。综合分析试验猪的病毒感染、临床表现和生产性能等情况表明Cap蛋白亚单位疫苗具有免疫保护效果,猪α干扰素可显著增强Cap蛋白亚单位疫苗接种仔猪的体液免疫反应,提高PCV-2 Cap蛋白亚单位疫苗免疫保护效果。
The immunogenicity of porcine circovirus type 2 （PCV2） Cap protein （rCap） expressed secretively by Pichia Pastoris and adjuvant effect of recombinant porcine interferon alpha （rPolFN-α） were examined in piglets in the present experiment. Groups of 30-day old piglets which were negative of PCV2 antibody were inoculated with recombinant Cap protein in the pres- ence or absence of rPolFN-α in a prime-boost protocol. Then the piglets were challenged with PCV2 JS strain at 14 days post the boost-vaccination. Neutralizing antibody against PCV2 was produced in piglets inoculated with Cap protein. The use of rPolFN-α resulted in significantly higher ELISA antibody and neutralizing antibody levels than recombinant Cap protein inocula- tion. After challenge, all control piglets developed viremia and had a long time fever, only one of four piglets receiving recombinant Cap protein of PCV2 developed viremia, while addition of rPolFN-α produced an improved protection to the viral challenge, since PCV2 replication was completely inhibited. As evaluated by viremia ratio, clinical signs （fever）, growth parameters, the piglets were protected against PCV2 challenge after vaccination of rCap. rPolFN-α showed potential adjuvant effects on recombinant Cap protein of PCV2, which was characterized not only in antigen specific humoral immune response, but also in protection against PCV2 infection.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第6期867-872,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家青年自然基金(30600018)
国家行业专项子课题(200803020-4)
南京农业大学青年科技创新基金(KJ05013)
