摘要
目的探讨血管内皮生长因子受体2（vascular endothelial growth factor receptor 2，VEGFR-2）在内皮祖细胞（endothelial progenitor cells，EPCs）血管新生中的作用。方法通过VEGFR-2小干扰RNA（small interfering RNA，siRNA）转染EPCs，检测其转染效率与抑制效率，观察EPCs体外血管生成以及迁移能力，并分析其基质金属蛋白酶-9（matrix metalloproteinases-9，MMP-9）、丝氨酸／苏氨酸激酶（serine threonine，Akt）、胞外信号调节激酶（extracellular signal—regulated kinase，ERK）的蛋白表达。结果VEGFR-2 siRNA能有效并特异地抑制EPCs VEGFR-2的表达；VEGFR-2 siRNA能抑制EPCs的体外血管生成与迁移能力；VEGFR-2 siRNA能抑制EPCs MMP-9的表达与活性，以及Akt与ERK的磷酸化水平。结论VEGFR-2 siRNA可能通过下调MMP-9、Akt与ERK的表达来抑制EPCs血管新生。
Objective To investigate the role of VEGFR - 2 in the angiogenesis of endothelial progenitor cells （EPCs）. Methods Tube formation, migration, VEGFR -2, Akt and ERK expression, and MMP -9 activity and expression were determined by tube formation assay on matrigel, migration assay, Western blot analysis, and gelatin zymography, respectively. Results VEGFR -2 siRNA inhibited VEGFR -2 expression in EPCs, cell migration and tube formation on matrigel. Moreover, MMP-9 activity and expression were decreased by VEGFR- 2 siRNA. The Akt and ERK phosphorylations were involved in EPCs angiogenesis mediated by VEGFR- 2. Conclusion VEGFR- 2 siRNA can inhibit EPCs angiogenesis via inhibition of MMP - 9 activity and expression, and down - regulation the Akt and ERK signal pathways.
出处
《中华神经外科杂志》
CSCD
北大核心
2009年第6期568-571,共4页
Chinese Journal of Neurosurgery
基金
江苏省卫生厅医学科技发展基金资助项目（H200510）江苏省医学重点人才项目（RC2007061）
