摘要
目的建立分辨率高和重复性好的侵袭性与非侵袭性垂体腺瘤的双向凝胶电泳图谱，并识别鉴定出其差异表达的蛋白质。方法利用固相pH梯度双向凝胶电泳分离侵袭性与非侵袭性垂体腺瘤的总蛋白质，凝胶经银染显色后，运用Image Master2D图像分析软件进行比较分析、识别差异表达的蛋白质，应用基质辅助激光解吸电离飞行时间质谱（MALDI—TOF—MS）及数据库搜索鉴定部分差异蛋白质点。结果得到了分辨率较高、重复性好的侵袭性与非侵袭性垂体腺瘤的双向凝胶电泳图谱。比较分析图谱，初步对其中30个点进行了肽质指纹图分析，鉴定出一些与细胞周期调控、信号传导等有关的差异蛋白质。结论建立了分辨率较高且重复性较好的侵袭性与非侵袭性垂体腺瘤的双向凝胶电泳图谱，并识别鉴定出一些侵袭性与非侵袭性垂体腺瘤的差异表达的蛋白质，为进一步筛选侵袭性垂体腺瘤的蛋白质表达数据库提供了基础。
Objective To analyze the differential protein expression by high resolution and reproducibility two - dimensional electrophoresis profiles in the invasion and noninvasion pituitary adenomas. Methods The proteome from the invasion and noninvasion pituitary adenomas tissues were separated and identified by using a series of methods, including immobilized pH gradient two - dimensional polyaerylamide gel electrophoresis, silver staining, lmageMaster 2 DE analysis software, peptide mass fingerprint based on matrix - assisted laser desorptiotr/ionization time of flight mass spectrometry （ MALDI - TOF - MS ） and database searching. Results The good 2 DE pattern of the invasion and noninvasion pituitary adenomas tissue including resolution and reproducibility were obtained. The map of the invasion pituitary adenomas tissues was compared with the noninvasion pituitary adenomas. Thirty proteins were analyzed, some of which have the relationship of oncogenes, and others were the regulation proteins, which adjust the cell cycle and signal transduction. Conclusion These data suggest that we constructed a high resolution and reproducibility of two - dimensional electrophoresis profiles from the invasion and noninvasion pituitary adenomas and analyze the differential protein spots, which will be taken advantage to establishing the differential proteomic expression profiles. These data will be used for establishing the invasion pituitary adenomas proteome database.
出处
《中华神经外科杂志》
CSCD
北大核心
2009年第6期504-507,共4页
Chinese Journal of Neurosurgery
基金
湖南省科技厅资助科研项目（No.06FJ3168）
