摘要
目的探讨乙型肝炎病毒(hepatitis B virus HBV)感染在原发性肝细胞癌(hepatocellular carcinomaHCC)发生中的病因学意义及其与survivin及Bcl-2蛋白表达的相关性及对HCC生物学行为的影响。方法利用血清学指标检测61例HCC的HBV感染情况,采用免疫组化检测癌组织中survivin及Bcl-2蛋白的表达。结果HBV感染组中survivin及Bcl-2蛋白表达率分别为69.23%(27/39)和71.97%(28/39);非HBV感染组中二者的阳性表达率为31.81%(8/22)和40.90%(9/22);分别有显著性差异(P<0.05)。Bcl-2及Survivin表达与门脉癌栓形成、肿瘤直经大小及AFP增高相关,与分化程度及肝内转移无关;BCL-2表达与Survivin表达正相关。在HCC中HBV感染与肿瘤的Edmondson分级、AFP增高、门静脉癌栓形成、肝内转移及转氨酶升高相关(P<0.05)。结论HBV感染可能与BCL-2及survivin共同作用导致HCC的发生及发展。
Objective To study the influences of HBV infection on biological behavious of hepatocellular cancinoma （HCC） and expression of surviving and Bcl-2 protein . Methods The state of HBV infection were determined by serum tests for 61 patients with hepatocellular cancinoma. SP immunohistochemical （IHC）methods were used detect the expreesions of Bcl-2 and survivin proteins in 61 hepatocellular carcinoma ,respectively. Results The positive expression rate of Bcl-2 and survivin were 71.97%（28/39） and （69.23% （27/39）） in HBV- positive group was significantly higher than of HBV-negative group（40.90%（9/13）, 31.81%（8/22）p〈0.05）. the expression of Bcl-2 and survivin was positively correlations with intraportal embolism ,the toumer size and the elevation of serum a-fectoprotien（AFP）levels,have no correlations with differentiation and intrahepatic metastasis, The expression of survivin significantly linked with mt-Bcl-2 . the statas of HBV infection is positively correlations with Edmondson gardes ,the elevation of serum α- fectoprotien（AFP）levels,intraportal carcinoma embolism,intrahepatic metastasis,and elevation of serum γ-glutamyl transferase（P 〈0.05）.Conclusion the state of HBV infection may join Bcl-2 and survivin protein in the occurrence and development of HCC.
出处
《医学信息》
2009年第7期1254-1256,共3页
Journal of Medical Information
