摘要
[目的]比较不同溶剂提取海金沙总黄酮对自由基活性清除能力的大小。[方法]应用DPPH法、邻苯三酚自氧化法和Fenton反应分别研究了浓度95%乙醇、乙酸乙酯、丙酮、乙酸、氯仿、甲醇提取物清除自由基活性的能力。[结果]6种溶剂提取得到的海金沙提取物对DPPH.、OH.和O2-.均有一定程度的清除作用,不同溶剂所得提取物对自由基的清除作用均有差别,其中浓度95%乙醇提取得到的海金沙提取物对3种自由基清除效果均最好。[结论]海金沙可作为抗氧化物质资源进行开发利用。
[ Objective ] The research aimed to compare the scavenging abilities of total flavone from Lygodium japonicum with different solvents to free radical activity. [ Method] DPPH method, pyrogallol method and Fenton reaction were used to study the scavenging ability of extracts with 95% ethanol, ethyl acetate, acetone, acetic acid, chloroform, methanol to free radical activity. [ Result] Six kinds of solvent extracts from L. japonicum had certain scavenging ability to DPPH ·, OH ·and O2^ -·. The scavenging ability of extracted with different solvents to free radicals were difference. The extracts from L. japonicum with 95% ethanol had the best scavenging effects on three kinds of free radicals. [ Conclusion ] L japonicum can be used as antioxidants for the development and utilization of resources.
出处
《安徽农业科学》
CAS
北大核心
2009年第19期8989-8991,共3页
Journal of Anhui Agricultural Sciences
基金
广东药学院博士启动基金(2007YKX12)
关键词
海金沙
黄酮
自由基
清除作用
Lygodiumjaponicum（Thunb）Sw
Flavone
Free radicals
Scavenging
