摘要
[目的]为杂种小麦加工品质预测和优质小麦亲本组配提供依据。[方法]以杂交小麦母本晋麦47和父本丰优1号及其F2籽粒为材料,采用SDS-PAGE电泳法对小麦籽粒中的高分子量麦谷蛋白亚基(HMW-GS)的分离及表达进行分析。[结果]F2籽粒中共出现21种HMW-GS带型组合,其中回归母本的带型(N、7+9、3+12)占69.10%,回归父本的带型(1、17+18、5+10)占1.04%,其余29.86%为双亲杂合带型或缺失带型;HMW-GS在F2籽粒中按一定规律进行分离,G lu-D lb,G lu-D ld出现基因重组(重组率5.90%),17+18和7+9亚基同时在G lu-B1位点表达,而G lu-B lc在该位点出现表达空位(空位率5.20%)。[结论]明确了HMW-GS在小麦F2籽粒中的表达及分离规律。
[Objeetive ] The aim was to provide the basis for forecasting processing quality of hybrid wheat and combining parents in high quality wheat breeding process. [ Method ] With grains of female parent Jinmai 47, male parent Fengyou 1 and F2 of hybrid wheat as the materials, the isolation and expression of high molecular weight glutenin subunits （HMW-GS） in grains were analyzed by SDS-PAGE electrophoresis. [ Result] 21 kinds of band phenotypes of HMW-GS appeared in F2 grains. Among which band phenotypes that regressed to female parent （ N, 7 ＋9, 3 ＋ 12） was 69.10% , and that regressed to male parent （ 1,17 ＋ 18,5 ＋ 10） was 1.04% , the remained 29.86% band phenotypes were hybrid band phenotypes and deletion band phenotypes.. HMW-GS isolated in F2 grains according to a certain inheritance, Glu-DIb and Glu-Dld was recombined （ recombination rate of 5.90% ）, 17 ＋ 18 and 7 ＋ 9 subunits expressed at GIu-B1 site at the same time, while there were no expression of Glu-Blc at this site （vacancy rate of 5.20% ）. [ Conclusion] This study determined the law of expression and isolation of HMW-GS in F2 grain of wheat.
出处
《安徽农业科学》
CAS
北大核心
2009年第19期8918-8919,共2页
Journal of Anhui Agricultural Sciences
基金
山西农业大学青年基金项目(2008012)
