摘要
采用摇瓶培养法对棕灰口蘑（Tricholoma terreum）适宜的发酵值碳源和氮源、发酵条件进行了分析研究，结果表明：最适宜的碳源、氮源分别是葡萄糖和酵母膏；最优发酵条件的初始pH值5．6-6．2，振荡速度160r／min，培养温度26℃，100ml三角瓶装液量60ml、发酵周期5d。此外，还测定了菌丝体与子实体中铁、锌、铜、锰4种矿质元素和蛋白质中的氨基酸组成。
Using flask culture method to study appropriate fermentation carbon and nitrogen source, fermentation conditions of Tricholoma terreum. The results showed that the most suitable carbon sources, nitrogen source were glucose and yeast extract. The optimal conditions of fermentation was the initial pH 5.6 to 6.2, hunting speed 160 r/min,culture temperature 26 ℃, 60 ml fluid in 100 ml triangle bottles,ferment 5 days. In addition, the author detected and analysed Fe,Zn, Cu, Mn four elements content and amino component of protein in mycelia and sporophores.
出处
《种子》
CSCD
北大核心
2009年第6期34-37,42,共5页
Seed
基金
贵州省自然科学基金资助项目[黔科合J字(2007)2047号]
中国高等教育学会"十一五"重点教育研究课题(06AIJ0860043)资助
关键词
棕灰口蘑
深层发酵
菌丝体
优化
成分分析
Tricholoma terreum
submerged fermentation
mycelia
optimzing
components analysis
