摘要
应用地高辛标记反意生长抑素ｃＲＮＡ探针原位杂交技术，研究５日龄ＡＡ肉鸡生长抑索ｍＲＮＡ阳性神经元胞体在海马结构及其皮质样层内的分布。结果表明：生长抑素ｍＲＮＡ阳性胞体主要分布在背侧海马及隔海马核内；此外，在背内侧海马，旁海马及其周围皮质内也有少量标记细胞。生长抑素ｍＲＮＡ阳性胞体为梭形和椭圆形，直径约７～２０μｍ。胞质内均有强度不等的黑色或蓝黑色杂交反应物。
Somatostatin(SS)mRNApositive neurons in the chicken hippocampal formation and its surrounding cortex were demonstrated by in situ hybridization histochemistry with digoxigenin labeled antisense SS cRNA probe.The results showed that SSmRNApositive neurons were fusiform or elliptical in shape and 7~20μm in size.These were different densities of hybridized reaction products in perikaryon.
出处
《中国兽医杂志》
CAS
北大核心
1998年第6期3-4,共2页
Chinese Journal of Veterinary Medicine
关键词
鸡病
生长抑素MRNA
海马结构
组织化学
broiler chickens
somatostaitn mRNA
hippocampus
in situ hybridization histochemistry
