摘要
对运动发酵单胞菌232B同步糖化发酵(SSF)鲜甘薯快速生产燃料乙醇的条件进行了研究.通过单因素试验和正交试验获得了乙醇发酵的最佳参数为:初始pH值6.0～7.0,硫酸铵5.0g/kg,糖化酶量1.6AUG/kg淀粉,初始总糖浓度200g/kg,接种量φ=12.5%.经过21h发酵,乙醇浓度为95.15g/kg,发酵效率可达94%.同时对不灭菌发酵也进行了研究,发酵效率可达92%.残糖的HPLC分析结果说明,发酵液中已没有葡萄糖存在,经酸水解后又出现了葡萄糖、半乳糖、甘露糖等成分,说明发酵结束后的残糖是多种低聚糖.
The ethanol production with simultaneous saccharification and fermentation （SSF） by Zymomonas mobilis 232B using fresh sweet potato as raw material was studied. The effects of various factors on the yield of ethanol were investigated with the single factor and the orthogonal experiments. As a result, the optimal technical conditions were obtained from those experiments： Initial pH value 6.0-7.0, nitride 5.0 g/kg （NH4）2SO4, glucoamylase 1.6 AUG/kg starch and inoculums φ=12.5%. The ethanol yield by Z. mobilis 232B reached 95.15 g/kg, with 94% of the theoretical yield, after 21 h fermentation. The nonsterilized fermentation was also studied and its yield of ethanol reached 92% g/kg. The analysis of residual sugars by HPLC showed that there was no glucose existing in the fermentation liquor. The glucose, galactose and mannose appeared after being hydrolyzed by acid. It is indicated that the residual sugars in the final fermentation liquor were oligosaccharides.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2009年第3期405-409,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家科技支撑计划项目(No.2007BAD78B04)
四川省科技攻关项目(No.027.X028-037)~~
关键词
鲜甘薯
运动发酵单胞菌
同步糖化发酵
乙醇发酵
fresh sweet potato
Zymomonas mobilis
simultaneous saccharification and fermentation （SSF）
ethanol fermentation
