摘要
[目的]研究速效救心丸对动脉粥样硬化(AS)大鼠模型基质细胞衍生因子(SDF-1)/趋化因子受体(CXCR4)表达的影响进而探讨其防治AS的机制。[方法]采用高胆固醇饮食加大剂量维生素D3腹腔一次性注射方法建立AS大鼠模型。60只SD大鼠随机分为6组:正常组,模型组,速效救心丸低、中、高剂量组,阿托伐他汀组。12周后取胸主动脉苏木—伊红(HE)、三色(MASSON)染色,用图像分析系统分析其病理改变,免疫组化法检测SDF-1及CXCR4蛋白表达情况。[结果]与模型组相比,速效救心丸中、高剂量组及阿托伐他汀组可明显减轻动脉内膜及中膜厚度,中药高剂量组还可抑制胶原纤维增殖,并降低胶原纤维在血管壁中的比例;各中药组及西药组均可下调SDF-1/CXCR4蛋白的表达。[结论]速效救心丸可明显拮抗AS大鼠病变动脉内膜及中膜增生,抑制胶原纤维增殖,从而延缓AS进程,其机制可能与抑制SDF-1/CXCR4轴功能有关。
[Objective] To investigate the effect of Suxiao Jinxin（improving the heart function with a high speed） Pellet on the expression of SDF-1/CXCR4 in rats with atherosclerosis and to find it＇s mechanism of anti-atherosclerosis. [Methods] Atherosclerosis was estab- lished in rats by feeding with atherogenic diet and intraperitoneal injecting with vitamin D3 as a bolus. Sixty SD rats were randomly divided into 6 groups： normal group（N）, AS model group（Mo）, Suxiao Jiuxin pellet low（SXL）, medium（SXM）, high（SXH） dosage group and As- torvastain group（ATO）. Twelve weeks later, the pathological change of Aortas was analyzed by Image Pro Plus system; SDF-1 and CXCR4 were examined by immunohistochemistry. [Results] Compared with Mo, the SXM, SXH and ATO could significantly reduce the arterial intima and media thickness, SMH could also inhibit the proliferation of the collagen fiber and decrease percentage in vessel wall; SXL, SXH,SXN and ATO could decrease the expression of SDF-1 and CXCR4; Between ATO,SXM and SXH there were no significant differ- ence （P〉0.05）. [Conclusion] Suxiao Jiuxin Pellet （medium, high dosage） can prevent the proceeding of AS by reducing the arterial thick- ness, inhibiting proliferation of the collagen fiber, delaying the process of AS.
出处
《天津中医药》
CAS
2009年第3期236-239,共4页
Tianjin Journal of Traditional Chinese Medicine
