摘要
以5-溴尿嘧啶为内标，建立了高效液相色谱法测定小鼠组织中肝靶向前药（半乳糖化人血清白蛋白与5-氟尿嘧啶偶联物）中5-氟尿嘧啶（5-Fu）含量的分析方法。样品经盐酸水解、乙酸乙酯提取、离心后，取上清液在40℃下用N2吹干，用流动相溶解、定容后进样分析。色谱条件：安捷伦Hypersil C18色谱柱（4．0mm×250mm，5μm），流动相为甲醇-磷酸缓冲溶液，流速0．7mL/min，UV检测波长为270nm。方法的线性范围为1～20mg／L，检出限（S／N=3）为0．1mg／L，回收率为93％～104％。该法操作简便、灵敏度高，适于组织中肝靶向前药半乳糖化人血清白蛋白与5-氟尿嘧啶偶联物（Gal—HSA-5-FU）的5-Fu含量测定。
5-Fluorouracil A high performance liquid chromatography method was developed for the determination of 5-fluorouraeil （5-FU） in galactosylated human serum albumin 5-fluorouracil conjugate （ Gal-HSA - 5-FU）, one kind of the hepatic target predrug, in mice tissues by using 5-bromine uracil（5-BrU） as internal standard. The sample was hydrolyzed by hydrochloric acid, extracted by ethyl acetate and separated by centrifugation. The supernatant was dried by nitrogen gas at 40 ℃, dissolved by methanol- phosphoric acid buffer. The chromatographic analysis was performed on an Agilent Hypersil C18 column（4.0 mm× 250 mm, 5 μm） with methanol -phosphoric acid buffer solution as mobile phase. The flow rate for mobile phase was 0. 7 mL/min and the detection wavelength was set at 270 nm. The linear range was 1 -20 mg/L with the detection limits of 0. 1 mg/L（ S/N = 3 ）, and the recoveries for the method were between 93% - 104% . The method was simple, sensitive and applicable to determine the 5-FU content of hepatic target predrug in tissues.
出处
《分析测试学报》
CAS
CSCD
北大核心
2009年第6期746-748,共3页
Journal of Instrumental Analysis
