摘要
目的观察高糖状态下，人类肾小球系膜细胞（ＧＭＣ）增殖，细胞内游离钙，纤维连接蛋白（Ｆｎ）及白介素－６（ＩＬ－６）的变化。方法采用体外ＧＭＣ培养，３Ｈ－胸腺嘧啶（３Ｈ－ＴｄＲ）掺入和细胞计数，Ｆｕｒａ－２双波长荧光法和ＥＬＩＳＡ法。结果高糖对ＧＭＣ增殖有抑制作用，对Ｆｎ及ＩＬ－６的生成有促进作用，而对细胞内游离钙水平无显著影响。
Objective To observe the effect of high glucose on the proliferation of cultured human glomerular mesangial cells,the level of cytosilic free calcium[Ca^2+]i,the fibronectin(Fn) and interleukin6(IL-6)levels in the tissue cultured supernatantMethods The culture of human glomerular mesangial cells was established and the cellular proliferation was assessed using 3 Hthymidine incorporation and cell counting,production of Fn and IL6 was analysed by ELISA,and the [Ca^(2+)]i level was determined by Fura2/AMResults High glucose depressed 3 HTdR incorporation while increased the production of Fn and IL6,but had no significant effect on [Ca^(2+)]i levelConclusion Hyperglycemia may be anentiologic factor of diabetic nephropathy
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
1998年第3期163-166,共4页
Chinese Journal of Nephrology
