摘要
目的:探讨交变磁场的物理作用与生物学效应的关系,研究磁场对小鼠睾丸生殖功能的影响。方法:30只ICR雄性小鼠随机均分为正常对照组、X线照射组、弱磁场(1000Hz)组、强磁场(2000Hz)1h组和强磁场2h组。照射后7d处死小鼠,分析附睾精子活率,对睾丸组织切片行HE染色观察睾丸病理组织变化,以Johnsen评分标准,评定睾丸变化积分。结果:精子活率正常对照组为(42.37±10.24)%,X线照射组为(39.00±12.35)%,弱磁场组为(36.00±17.28)%,强磁场1h组为(10.72±5.67)%,强磁场2h组为(4.44±2.87)%,强磁场1h组和2h组与正常对照组比较,精子活率明显下降(P<0.01)。睾丸病理变化Johnsen评分随着交变磁场的加大和时间延长积分越低,睾丸损伤越严重。结论:强、弱磁场均引起小鼠睾丸功能破坏,导致生精小管损伤,间质、间质细胞损坏,基膜增厚,腔内生精细胞排列紊乱,凋亡、坏死和脱落增多,导致无精子发生。
Objective： To explore the relationship between physical and biological effects of alternating magnetic field and study the influence of the magnetic field on the reproductive function of murine testes. Methods ： Thirty ICR mice were randomized into 5 groups ： normal control, X-ray radiation, weak magnetic field （ 1 000 Hz ） , 1 h strong magnetic field and 2 h strong magnetic field （2 000 Hz）. The mice were sacrificed at 7 days after the exposure for the analysis of testicnlar sperm motility, observation of histopathological changes in the testis by HE staining and evaluation of the changes by modified Johnsen grade criteria. Results： The rates of sperm motility were （42.37 ± 10.24）% in the normal control group, （39.00 ± 12.35 ）% in the X-ray radiation group, （36. 00 ± 17.28） % in the weak magnetic field group, （ 10.72 ± 5.67） % in the 1 h strong magnetic field group and （4.44 ± 2.87 ） % in the 2 h strong magnetic field group, respectively. Johnseng scores decreased and the testis damage increased in a dose- and time-dependent manner. Conclusion ： Magnetic field, either strong or weak, may damage the testis function by inducing injury to seminiferous tubules and Leydig ceils, thickening of the basal membrane, derangement, exfoliation, massive apoptosis and necrosis of spermatogenic cells in the lumen, siltation of the epididymis, and consequently the absence of sperm.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2009年第6期530-533,共4页
National Journal of Andrology
关键词
交变磁场
精子
生精细胞
睾丸
生殖功能
小鼠
alernating magnetic field
spermatozoon
spermatogenic cell
testis
reproductive function
murine
