摘要
目的:筛选与精子活力相关的基因。方法:将活力正常者与弱精子症者精子cDNA与Affymetrix全基因组芯片进行杂交,筛选出差异表达基因。RT-PCR分析该基因在人体不同组织及精子中的表达,并比较该基因在活力正常和活力低下的人精子中表达的差异。结果:芯片结果分析筛选出差异表达基因:电压依赖性阴离子通道(VDACs)基因。VDACs包括VDAC1、VDAC2、VDAC33个亚型,它们均在人精子中表达。与活力正常组精子VDAC2相对表达量(0.803±0.043)比较,VDAC2在弱精子症组精子的相对表达量(0.568±0.036)显著降低(P<0.01)。结论:VDAC2的表达减少可能与精子中活力降低有关。
Objective： To identify the genes involved in sperm motility. Methods： We hybridized asthenospermial and normal motile sperm cDNA samples with the human whole genome Affymetrix chip to screen differentially expressed genes. Then we detected the mRNA expressions of the voltage-dependent anion channel genes （VDACs） in human organs and spermatozoa by RT-PCR and compared their expressions in the poor and normal motility spermatozoa. Results： Differentially expressed genes VDACs were identified by anal- ysis of the hybridization signals, including the 3 subtypes VDAC1, VDAC2 and VDAC3. The expression of VDAC2 mRNA was significantly decreased in the poor motility sperm （0. 568 ＋ 0. 036）, as compared with the healthy men （0. 803 ± 0. 043, P 〈 0.01 ）. Conclusion： The decreased expression of VDAC2 in the ejaculated spermatozoa is possibly associated with the reduction of sperm motility.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2009年第6期493-498,共6页
National Journal of Andrology
基金
国家自然科学基金(30770810)
广东省科技计划项目(2007B060401061)
深圳市科技计划项目(200602059)~~
关键词
基因芯片
弱精子症
电压依赖性阴离子通道
逆转录-多聚酶链反应
gene chip
asthenospermia
voltage-dependent anion channel
reverse transcription polymerase chain reaction
