摘要
目的:将乳腺癌MDA-MB-231细胞的SK-1基因敲除,结合阿霉素对细胞增殖和迁移的抑制作用,研究乳腺癌的治疗新方法。方法:将阿霉素分别感染野生型及SK-1敲除型MDA-MB-231细胞,3H-TdR掺入法分析细胞增殖,RT-PCR检测细胞内SK-1的mRNA表达量,Western blot检测SK-1蛋白表达及细胞凋亡相关因子caspase 3的蛋白表达,Transwell法分析细胞迁移。结果:阿霉素感染MDA-MB-231细胞,抑制细胞增殖和迁移,细胞存活率明显下降;阿霉素呈浓度依赖性及时间依赖性抑制SK-1 mRNA及蛋白水平表达;将SK-1基因敲除,细胞迁移率下降,协同阿霉素的作用,迁移率进一步下降,且将导致细胞凋亡。结论:SK-1基因敲除可增强阿霉素对乳腺癌细胞增殖和迁移的抑制作用。
Objective： SK - 1 knockout in human breast cancer cell line MDA - MB - 231, cooperating with the inhibition of cell proliferation and migration by doxorubicin, to investigate new therapy for breast cancer. Method： The cell proliferation activities were measured by 3H-TdR and the cell migration was performed by Transwell. Western blot was used to detect SK- 1 expression and caspase 3 expression and RT-PCR was performed to observe SK - 1 mRNA. Result： Doxorobicin inhibited the proliferation and migration of human breast cancer cell MDA - MB - 231 and down - regulated SK - I mRNA and protein expression in a dose- and a time - dependent manner. The cell migration was inhibited by SK - 1 knockout. The SK- 1 knockout cells infected by doxorubicin induced apoptosis. Conclusion： SK- 1 knockout may promote the inhibition of proliferation and migration of MDA - MB - 231 cells by doxorobiein.
出处
《生物技术》
CAS
CSCD
北大核心
2009年第3期22-24,共3页
Biotechnology
