摘要
目的研究系统性红斑狼疮（SLE）患者外周血CD4^＋CD39^＋T细胞中FOXP3蛋白的表达情况，以及糖皮质激素治疗的影响。方法采用流式细胞术检测47例SLE患者（其中29例为初发未经治疗的活动期SLE）和22名正常人外周血CD4^＋CD25^＋CD39^＋T细胞、CD4^＋CD25^＋FOXP3^＋T细胞及CIM^＋CD39^＋FOXP3^＋T细胞百分率以及FOXP3蛋白的表达，分析3组细胞之间的相关性及糖皮质激素治疗的影响。结果SLE活动组、缓解组、正常对照组外周血CD4^＋CD25^＋CD39^＋T细胞百分率分别为（1．3±0．5）％、（1．9±0．8）％、（2．3±1．0）％，该群细胞在SLE活动组中的表达水平低于缓解组和正常对照组（均P〈0．05），而在后2组之间差异无统计学意义（P〉0．05）；SLE活动组中CD4^＋CD25^＋、CD4^＋CD25^high及CD4^＋CD39^＋T细胞表达的FOXP3蛋白百分率分别为（45±12）％、（65±14）％、（70±14）％，FOXP3蛋白在CD4^＋CD39^＋T细胞和CD4^＋CD25^high T细胞中的表达水平明显高于在CD4^＋CD25^＋T细胞中的表达水平（P〈0．01），而在CD4^＋CD39^＋T细胞与CD4^＋CD25^high T细胞中的表达水平差异均无统计学意义（均P〉0．05）。结论CD39可能是调节性T细胞较好的表面标记，CD39^＋Treg细胞表达异常可能参与SLE的发病机制。
Objective To investigate the level of FOXP3 expressed in CD4^ ＋ CD39 ^＋ T cells in peripheral blood of patients with systemic lupus erythematosus （SLE） and observe the regulation of glucocorticoid on it . Methods Frequencies of CD4 ^＋ CD25 ^＋ CD39 ＋ , CD4^ ＋ CD25 ^＋ FOXP3^ ＋ and CD4^ ＋ CD39 ^＋ FOXP3^ ＋ T cells and levels of FOXP3 protein were analyzed by flow cytometry of 47 SLE patients （ including 29 untreated/active SLE） and 22 healthy controls. Meanwhile, correlations among three groups and influences of glucocorticoid were analyzed. Results Percents of CD4^ ＋ CD25 ＋ CD39 ＋ T cells expressed in active SLE, inactive SLE and healthy controls were （ 1.3 ± 0.5 ） %, （ 1, 9 ±0.8 ） % and （2.3±1.0） % respectively, the level decreased in active SLE compared with inactive SLE and healthy controls P 〈 0.05 in each group, but it had no significant difference between the latter two groups （ P 〉 0.05 ）. In active SLE, levels of FOXP3 protein expressed in CD4 ^＋ CD25^ ＋ , CD4^＋ CD25^high and CD4^＋ CD39^＋ T ceils were（45 ± 12 ） % , （65 ± 14 ） % and （ 70 ± 14） % respectively. Levels of FOXP3 expressed in CD4 ^＋ CD25^ high and CD4^ ＋ CD39 ^＋ T cells were higher than that expressed in CD4^ ＋ CD25 ^＋ T cells （ P 〈 0. 01 ）, while it had no significant difference between CD4^ ＋ CD25^high T cells and CD4 ^＋ CD39^ ＋T cells （P 〉 0.05 ）. Conclusions These results demonstrate that CD39 may be a better surface marker of regulatory T cells, and that deficiency of CD39 ^＋ Treg cells may play an important role in the pathogenesis of SLE.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2009年第23期1636-1638,共3页
National Medical Journal of China
基金
省临床医学重点学科应用技术项目（05A008）
安徽省国际合作项目（07080703022）
