摘要
由最终产物为邻苯基苯酚(2-HBP)的二苯并噻吩(DBT)的4-S代谢途径出发,从被高硫原油污染的土样中分离,纯化得到一株能高效降解DBT的菌株,通过形态学,生理生化试验及16S rDNA基因测序,归类为Mycobacterium sp.对细菌的培养条件进行研究,初步确定较为适宜的培养条件:温度为40℃,pH值为7.0,转速为200r/min.在此培养条件下,利用该菌株处理含有5mmol/LDBT的正十二烷模拟相,24h以后,DBT减少到3.36mmol/L,平均比脱硫率为8.34mmol DBT h-1kg-1DCW(干细胞重)。
A new desulfurizing strain was isolated from an oil-contaminated soil and could decompose dibenzothiophene （DBT） into 2-hydroxybiphenyl （2-HBP） via a sulfur specific path way. The strain was identified as Mycobacterium sp. by the analyses of morphology, physiology, and 16S rDNA sequence. The optimum culture conditions for the growth of the strain were determined as follows： temperature at 40℃, pH 7.0 and the stirring speed of the shaker 200 r/min. Under these conditions, the strain could decompose DBT in the n-hexadeeane solution from 5mmol/L to 3.36 mmol/L in 24 h, with average desulfurization rates of 8.34 mmol DBT h^-l kg^-1 DCW （dry eell weight）.
出处
《工业微生物》
CAS
CSCD
2009年第3期34-39,共6页
Industrial Microbiology
关键词
柴油
分离
鉴定
生物脱硫
二苯并噻吩
diesel
isolation
identification
bio-desulfurization
dibenzothiophen
