摘要
目的探讨非特异性金属基质蛋白酶抑制剂多西环素（Doxycycline）对高氧肺损伤肺形态学的影响。方法新生SD大鼠生后12h内随机分为4组：空气＋生理盐水组（AN组）、空气＋多西环素组（AD组）、高氧＋生理盐水组（ON组）、高氧＋多西环素组（OD组）。高氧组（ON、OD组）大鼠于90％氧气条件下饲养。多西环素组（AD、OD组）每天早晚两次经胃管喂入多西环素20mg／（kg·次），喂药至实验日或生后14d。1、3、7、14及21d进行肺组织切片的体视学研究。结果高氧及多西环素均导致平均肺泡面积增加，14d OD组星体积高于同期ON组。除ON组外，各组肺泡间隔均逐渐增厚，氧气和多西环素共同作用增加肺泡间隔厚度，且作用持续。单纯高氧使肺间质胶原含量明显增加；高氧合并多西环素作用早期胶原含量较AN组增加明显，14d后胶原含量降低并与AN组相近。结论高氧和（或）多西环素均影响肺发育，多西环素可减少高氧导致的肺间质中胶原比例增加。
Objective To explore the influence and potential protective effects of non-specific panMetrix Metalloproteinases （MMPs） inhibitor Doxycycline on hyperoxic lung injury and lung development in neonatal rats. Methods Neonatal SD rats were divided into 4 groups randomly within 12 hours after birth： air with normal saline （AN） ,air with doxycycline （AD） ,hyperoxia with normal saline （ON） ,and hyperoxia with doxycycline group （OD）. Hyperoxia groups （ ON and OD） were exposed to ≥90% O2. Doxycycline 20 mg/kg or equal volume of 0. 9% saline （ Doxycycline concentration 2 mg/ml） was administered by gastric gavage ,twice daily from day 1 to experimental day ,but it didn＇t exceed 14 days. Stereological study was carried out at day 1,3,7,14 and 21 after birth. Results Enlarged mean alveolar area was noted both in hyperoxia and Doxycycline groups. OD group had larger star volume than ON group on day 14. Except ON group, all other groups had increased alveolar septum and the condition was worsened by hyperoxia with Doxycycline. The percentage of collagen in lung parenchymal tissue in ON group increased persistendy. This increasing trend was stopped in OD group on day 14, and collagen percentage had no significantly difference between OD and AN groups on day 14 and 21. Conclusion Both hyperoxia and Doxycycline influence neonatal lung development, percentage of collgen in lung parenchymal can be reduced by Doxycycline in hyperoxic lung injury.
出处
《中国小儿急救医学》
CAS
2009年第3期256-259,共4页
Chinese Pediatric Emergency Medicine
关键词
新生鼠
肺损伤
体视学
高氧
多西环素
Rats, neonatal
Lung injury
Stereology
Hyperoxia
Doxycycline
