摘要
利用一株具有自身絮凝能力的粟酒裂殖酵母（Ｓｃｈｉｚｏｓａｃｃｈａｒｏｍｙｃｅｓｐｏｍｂｅ）变异株作为进行无载体固定化细胞酒精发酵的出发菌株，对影响其絮凝的环境因子和诱导操作条件进行了研究，并利用响应面分析技术（ＲＳＡ）对此菌株的絮凝诱导操作工艺进行优化，建立了经验模型和快速高效絮凝细胞颗粒技术的最佳工艺条件。
Inducing yeast cells to selffloculate as a cell immobilization method, the environmental factors and manipulative conditions of the flocs from Schizeosaccharomyces pombe were studied. Based on the results of screening experiment, glucose,peptone and shaking time were decided as the important induced factors. Using Respone Surface Analysis method, the suitable cultivated condition of the flocs was obtained. Under the optimal condition, the cell in flocs(%) induced in flask by S.pombe was 90% and the volume of flocs in culture (50 ml) was 5.2 ml.
关键词
粟酒裂殖酵母
絮凝颗粒
诱导
酵母
Schizeosaccharomyces pombe
flocs
inducing
RSA
optimitzation
