摘要
目的初步研究microRNA-21(miR-21)在大鼠胚胎植入过程中的作用。方法利用Northern印迹和原位杂交分析了miR-21在大鼠胚胎植入前期和植入期的表达差异,检测miR-21在假孕、人工诱导蜕膜化和延迟着床激活大鼠子宫中的表达模式,分析了17β-雌二醇和孕酮对于大鼠子宫miR-21表达的影响。结果 miR-21在植入前期和植入期的大鼠子宫中存在差异表达,在大鼠妊娠第5天miR-21的表达量开始增加,其主要分布在植入位点内腔基质细胞,在第5.5天子宫miR-21的表达量达到最高。在假孕的D4～D7大鼠子宫中miR-21表达无差异,在延迟着床激活模型中大鼠子宫miR-21的表达量明显增加,miR-21在诱导蜕膜化的大鼠子宫中表达也显著增加,17β-雌二醇和孕酮可以抑制大鼠子宫miR-21的表达。这些结果提示在植入期miR-21的表达增加主要是由胚泡引起的,并且其参与了蜕膜化过程。结论在大鼠妊娠早期,miR-21的表达增加可能有利于胚胎植入的发生。
Objective： To study the expression and regulation of miR-21 in the process of embryo implantation of rats. Methods： The differential expressions of miR-21 during the pre-implantation and implantation period and the expression profile in uterus of pseudopregnancy, artificial decidualization, delayed-implantation rats were detected by Northern blot and in situ hybridization. The effects of estradiol-17β or progesterone on miR-21 expression in uterus of ovariectomized rats were also investigated. Results： MiR-21 was highly expressed in the subluminal stromal cells at implantation sites on day 5 of pregnancy. The miR-21 expression was not significantly changed in uterus of pseudopregnancy rat and significantly increased in the uterus of artificial decidualization and delayed-implantation rat. The miR-21 expression was inhibited by treatment of estradiol-17β or progesterone. Collectively, the results suggested that increased miR-21 expression was mainly induced by blastocyst and may involve decidualization during the window of implantation, which may be beneficial to embryo implantation in the early of pregnancy in rats. Conclusion： MiR-21 may play an important role during embroy implantation in the rats.
出处
《生殖医学杂志》
CAS
2009年第3期284-290,共7页
Journal of Reproductive Medicine
基金
国家自然科学基金资助项目(30800396)
国家重点基础研究973计划资助项目(2007CB511905)
