摘要
Ｓｔｉｍｕｌａｔｉｏｎｏｆｃｅｎｔｒａｌｃｈｏｌｉｎｅｒｇｉｃｎｅｕｒｏｎｓｂｙ（－）ｃｌａｕｓｅｎａｍｉｄｅｉｎｖｉｔｒｏ１ＤＵＡＮＷｅｎＺｈｅｎ，ＺＨＡＮＧＪｕｎＴｉａｎ２（ＤｅｐａｒｔｍｅｎｔｏｆＰｈａｒｍａｃｏｌｏｇｙ，Ｉｎｓｔｉｔｕｔｅ...
AIM: To study the neurotrophic effects of (-) and (+)clausenamide on frontal cortex neurons in culture. METHODS: The activity of the choline acetyltransferase (ChAT) was determined by spectrophotometric method; protein content was assayed by Folin phenol method. RESULTS: (-)Clausenamide increased the activity of ChAT and protein content in cultured neurons, as well as stimulated proliferation of neuronal cells, support survival and neurite outgrowth of neurons. The neurotrophic action of (-)clausenamide (0 001-10 μmol·L -1 ) was similar to that of nerve growth factor. The (+)clausenamide had no neurotrophic action, even at high concentrations (0 1-10 μmol ·L -1 ), but neurons were damaged. CONCLUSION: (-)Clausenamide stimulated central cholinergic neuron development.
出处
《中国药理学报》
CSCD
1998年第4期332-336,共5页
Acta Pharmacologica Sinica
关键词
黄皮酰胺
胆碱乙酰转移酶
神经元
胆碱能神经元
clausenamide
cultured cells
choline acetyltransferase
atarabine
frontal lobe
cerebral cortex
