摘要
目的：研究促凋亡基因Ｂａｘ在膀胱移行细胞癌（ＴＣＣ）中的表达，探讨Ｂａｘ在ＴＣＣ发生、发展中的作用和预后复发中的意义。方法：采用兔抗人Ｂａｘ多克隆抗体及Ｓ－Ｐ免疫组化法检测。结果：５６例ＴＣＣ中有３４例Ｂａｘ蛋白表达阳性，阳性率６０．７％。２０例正常膀胱粘膜中有６例表达阳性，阳性率３０％。两组差异显著（Ｐ＜０．０５）。Ｂａｘ蛋白表达部位在细胞胞浆中。Ｂａｘ蛋白表达与病理分级、临床分期及肿瘤复发无显著相关性。结论：促凋亡基因Ｂａｘ在大部分ＴＣＣ中具有不同程度的表达与其发生有关，与ＴＣＣ分级、分期及复发无关。
Objective:We studied the expression and significance of proapoptotic gene Bax and its role in the genesis, progression and recurrence of the bladder transitional cell carcinoma(TCC). [WT5”HZ]Methods:Streptavidinperoxidase(SP)immunohistochemical and microwave antigen retrieval methods, polyclonal antibody against Bax protein on archival material were employed. Results:[WT5”BZ〗Bax immunostaining was observed in 34 out of 56 cases of bladder TCC and 6 out of 20 cases of normal control. The positive labeling was located in the tumor cell cytoplasm. Expression of Bax in bladder TCC was higher than that in normal control(P<0.05).Statistical analysis did not show a close correlation between Bax expression and pathologic grading, clinical staging and prognosis,respecoivety.[WT5”HZ〗Conclusions: Proapoptotic gene Bax differently expressed in most of bladder TCC,and significantly correlated with the tumorigenesis. Bas protein expression had no correlation to pathologic grading, clinical staging and prognosis,respectively.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1998年第2期178-180,共3页
Journal of China Medical University
关键词
膀胱肿瘤
免疫组织化学
促凋亡基因
bladder transitional cell carcinoma
proapoptotic gene Bax
immunohistochemical staining
