摘要
目的：探讨Ｂａｔｒｏｘｏｂｉｎ等药物对兔静脉移植模型早期ＰＣＮＡ表达的影响。方法：兔颈静脉移植于颈动脉后应用Ｂａｔｒｏｘｏｂｉｎ、ＬＭＷＨ、Ｕｒｏｋｉｎａｓｅ和Ｖｅｒａ－ｐａｍｉｌ治疗，并于不同时间采用免疫组化方法观察血管平滑肌（ＶＳＭＣ）中ＰＣＮＡ表达的变化。结果：术后３、７、１４ｄ均有ＰＣＮＡ的表达；３ｄ时Ｕｒｏｋｉｎａｓｅ、ＬＭＷＨ两组ＰＣＮＡ的表达与对照组无显著差异；Ｖｅｒａｐａｍｉｌ及Ｂａｔｒｏｘｏｂｉｎ两组的ＰＣＮＡ指数均低于对照及其他两组：术后７、１４ｄＶＳＭＣ中的ＰＣＮＡ各组的表达均与术后３ｄ相同只是强度略有下降；移植静脉ＰＣＮＡ指数均略低于相应动脉。结论：Ｕｒｏｋｉｎａｓｅ、ＬＭＷＨ对血管移植后ＶＳＭＣ内的ＰＣＮＡ无影响。
Objective: To investigate the influence of Batroxobin and other drugs on expression of proliferating cell nuclear antigen in early transplant vessels of Newzealand rabbit model with jugular vein transplanted to carotid. Methods: With the techniques of pathological, immunohistochemical to detect the expression of vascular smooth muscle cell in vein grafts and join arteries. Results: There was rather active PCNA expression on 3 d, 7 d and 14 d after venous graft, especially for 3 d. Urokinase and LMWH had no effect on PCNA expression in VSMC, but there was a significant inhibitory effect in Batroxobin and Verapamil groups compared with the three groups of contrast, Urodinase and LMWH. Conclusions: Batroxobin and Verapamil can inhibit the PCNA expression of vein grafts and join arteries in different time.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1998年第3期267-269,280,共4页
Journal of China Medical University
基金
卫生部科研基金
关键词
静脉移植
血管平滑肌细胞
增殖细胞核抗原
vein graft
proliferating cell nuclear antigen
vascular smooth muscle cell
