摘要
萤火虫荧光素酶只在萤火虫体内产生，一般通过养殖和用化学法提取。研究了以基因工程菌Ｅｓｃｈｅｒｉｃｈｉａｃｏｌｉ－Ｌｕｓ－Ｊ－１为材料，从它的对数生长期发酵液中提取萤火虫荧光素酶。经检测其分子量为５×１０４，动力学性质研究表明它是非典型的Ｍｉｃｈａｅｌｉｓ－Ｍｅｎｔｅｎ氏酶，对荧光素作用动力学曲线为Ｓ型，最适ｐＨ为７．５，最适温度为１５℃，且对光和盐浓度非常敏感。
The luciferase can be produced in body and can be obtained by breeding firefly and chemical extract. The luciferase has been purified from fermentative liquid of Escherichia coilLusJ, genetic recombination strain. Its molecular weight is 50000. Dynamic studies demonstrated that it does not behave the character of typical MechaelisMenten enzyme. The dynamics curve of the luciferase for luciferin shows a S shape. Its optimal pH and temperature are 7.5 and 15℃, respectively. It is sensitive to light and salt concentration.
出处
《清华大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
1998年第6期16-19,共4页
Journal of Tsinghua University(Science and Technology)
关键词
萤火虫
荧光素酶
分离
纯化
特性
firefly luciferase
separation and purification
property analysis
