摘要
建立了流动注射和光纤光度法测定肌酸激酶（Ｃｋ）活性的方法．求测了Ｃｋ催化反应的米氏常数ｋｍ和反应活化能Ｅ，研究了磁场对Ｃｋ催化活性的影响．较长时间磁化Ｃｋ溶液和测活溶液，分别可使Ｃｋ加速失活和活性升高，１００ｍＴ磁场磁化处理测活液１５ｈ可使Ｃｋ催化反应速度增加４３．７％．
Rapid and simple flow injection spectrophotometric and fiberoptic spectrophotometric method have been estabilished for the assay of Ck activity.The michaelis constant and activity energy of enzymic reaction were also determined.The effect of magnetic field on activity of Ck was studied.When the Ck is exposed to magnetic field for a long time,the activity of Ck is decreased accelerately.However,after long magnetizing the substrate solution the activity of Ck is increased.When the substrate solution is exposed to 100 mT magnetic field for 15 h,the rate of the enzymic reaction is increased by 43.7 %.No changes in the rate of the enzymic reaction were observed for periods of exposure of the reaction system in magnetic field.
出处
《湖南大学学报（自然科学版）》
EI
CAS
CSCD
1998年第3期31-35,共5页
Journal of Hunan University:Natural Sciences
基金
国家自然科学基金
关键词
肌酸激酶
催化活性
磁场
酶催化剂
creatine kinase(Ck),catalytic activity,magnetic field
