摘要
以北方春大豆主栽品种绥农14的干种子为材料,分别进行航天搭载和低能离子束注入处理。其中,航天搭载在实践八号返回式卫星上完成,离子束注入采用能量为30keV的氮离子,设置4×1016、8×10161、2×1016和16×1016N+/cm2等4个注量水平,在相同种植条件下对航天搭载和低能离子束注入处理M1代的生物学效应和M2代的变异频率进行研究,以比较两种诱变育种手段诱变效应的异同。结果表明,航天搭载处理M1代的出苗率、株高、单株荚数、单株粒数等与注量分别为4×1016和8×1016N+/cm2的离子束注入处理无显著差异。航天搭载处理M2代的总变异频率为4.41%,离子束注入处理M2代总变异频率的平均值为5.02%,两种处理间差异不显著;两种处理M2代中均有早熟、不育、多小叶、矮化、畸形茎、圆形叶片等变异类型,这些相同变异类型的总频率占航天搭载总变异频率的比例为97.3%,占离子束辐照处理总变异频率的比例为89.0%。鉴于两种处理的诱变频率和突变类型相近,但航天搭载的成本高,建议在大豆诱变育种中,可考虑选用离子束注入。
In the current studies, soybean seeds were treated with space flight and ion beam implantation, and their mutagenic effects were compared. Dry seeds of ＂Suinong 14＂, a widely-grown northern spring-sowing soybean variety, were carried by recovered satellite, Shijian-8, special designed for plant breeding, and by N^＋ beam implantation with the energy of 30 keV at four flux levels of 4 × 10^16 , 8 × 10^16, 12 × 10^16 and 16 × 10^16 N^＋/cm^2 . And the biological effects of the two treatments in M, generation and the variation frequency in M2 generation were compared under the same planting conditions. The results indicated that there was no significant differece for seed germination rate, plant height, pod and seed numbers per plant between space flight and ion beam implantation treatments at 4 × 10^16 and 8 × 10^16 N ^＋/cm^2 flux levels. The variation frequency in M2 generation of space flight treatment was 4.41%, and the average variation frequency was 5.02% for ion beam implantation, and there was no significant difference of variation frequency in M2 between the space flight and beam implantation. Mutants with early maturity, sterility, multi-leaflet, dwarf, deformed stem or round-shaped leaf, which totally accounted for 97.3 % in space flight treatment, and 89.0 % in average of 4 ion beam implantation treatments, were all found in the two different treatments. Since the variation frequency and variant types of the two treatments were similar, but space flight is more costly, therefore, it was proposed that ion beam implantation could partially substitute the space flight mutation in soybean breeding.
出处
《核农学报》
CAS
CSCD
北大核心
2009年第3期395-399,共5页
Journal of Nuclear Agricultural Sciences
基金
“863”(2006AA100104-9)
“973”(2002CB111300)
国家科技支撑计划(2006BAD01A04)
现代农业产业技术体系建设专项(cytx-004)
关键词
航天搭载
离子束注入
诱变
大豆
space flight
ion beam implantation
mutagenic effect
soybean
