摘要
发光二极管诱导荧光检测器(LED-IF)是近十年来发展起来的一种微型化荧光检测器,在流动注射、毛细管液相色谱、毛细管电泳及芯片电泳等微流动分析系统中具有广泛的应用。本文讨论了LED-IF的4种光学结构,并对其主要器件,包括光源、滤光片、透镜、光纤、光电检测器以及检测池作了详细讨论,还介绍了LED-IF与其它技术的联用及其在生物、医药和环境样品检测中的应用,对未来的发展趋势作了展望。
Light emitting diode induced fluorescence detector （LED-IF） has been developed and widely used as detectors in capillary flow systems, including flow injection analysis, capillary liquid chromatography, capillary electrophoresis and microchip analysis. This review summarizes the progress of LED-IF in recent years. Four kinds of optical arrangements are presented. The main parts of LED-IF, including light source, filter, lens, optical fiber, photo electronic detector and detection cell, are discussed in detail. The applications of LED-IF in bioanalysis, pharmaceutical analysis and environmental analysis are presented. Furthermore, the trend of development of LED-IF is also discussed.
出处
《化学进展》
SCIE
CAS
CSCD
北大核心
2009年第6期1325-1334,共10页
Progress in Chemistry
基金
国家自然科学基金项目(No.20227501)
中国科学院重大科研装备研制项目(No.YZ0605)
海洋监测高性能传感器技术项目(No.SQ2007AA09Z134007)资助
关键词
微型化
发光二极管
荧光
检测器
微流动分析
instrument miniaturization
light emitting diodes
fluorescence
detectors
capillary flow systems
