摘要
目的：建立黑鳗藤药材中黑鳗藤新苷K的含量测定方法。方法：应用反相高效液相色谱法测定黑鳗藤中黑鳗藤新苷K的含量,色谱柱使用D iamonsil C-18（250mm×4.6mm,5μm）;流动相为乙腈：水（48∶52）;检测波长为355nm;流速1mL/m in。结果：黑鳗藤新苷K在进样量2.024~14.168 mg范围内线性关系良好,回归方程为：Y=144701X-9335.5,r=0.9998;平均回收率为98.69%,RSD为1.48%（n=9）。结论：该方法简便,灵敏,准确,重现性好,可用于黑鳗藤药材的含量测定和质量控制。
Objective： To establish a method for determination of the content of stemucronatoside K in the roots of Stephanotis mucronata. Methods： The analysis was carried out by RP - HPLC on Diamonsil C - 18 （250mm × 4.6 mm, 5um）, acetonitrile：water （48：52） as mobile phase; wave length was 355 rim; flow rate was 1.0 mL/min. Results： The liner range of stemucronatoside K was 2.024 - 14.168mg. Regression equation was ： Y = 144701X - 9335.5, r = 0. 9998. The average recovery was 98.69% with RSD = 1.48% （n = 9）. Conclusion： The method is simple and accurate with a good reproducibility and can be used as a quantitative analysis for the roots of Stephanotis mucronata.
出处
《中华中医药学刊》
CAS
2009年第6期1260-1262,共3页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省自然科学基金资助项目(R206439)
浙江省医药卫生科技计划资助项目(2006QN004)
