摘要
目的考察不同剂量、不同给药时间、不同给药途径和不同方法制备的石花制剂对小鼠急性辐射损伤的防护作用,探讨石花制剂治疗小鼠急性辐射损伤的最佳给药方案。方法小鼠在照射前腹腔注射10mg/kg、20mg/ kg、40mg/kg、80mg/kg石花制剂,进行^(60)Coγ射线一次性全身照射,照射剂量为9Gy,观察小鼠的30d活存率和保护系数;照前15min、30min、1h、2h、4h腹腔注射石花制剂,观察30d活存率和保护系数;照前肌肉注射和口服给予石花制剂,观察30 d活存率和保护系数;照前腹腔注射醇提和水提的石花制剂,观察30d活存率和保护系数。结果石花制剂在一定剂量范围内(20～80 mg/kg)可显著提高受照小鼠的30d活存率和平均活存天数;照前15min至1h腹腔注射石花制剂对受^(60)Coγ射线照射小鼠的辐射损伤均有显著的治疗作用;照前肌肉注射石花制剂时,防护效果略逊于腹腔注射给药,而同样剂量口服给药时,则防护作用不显著;水提取的石花制剂在照前不同时间腹腔注射给药,防护效果与50%乙醇提取的石花制剂近似。结论石花制剂对小鼠急性辐射损伤有一定的防护作用,最佳给药方案为照前30min腹腔注射40mg/kg的石花制剂。
Objective To investigate the radiation protection effect of Parmelia tinctorum preparations in dosage, drug administration time, administration methods and different preparation methods on acute radiation -injured mice, and find out the best therapeutic project. Methods Kunming mice were injected （ip） with 10 mg/kg, 20mg/kg, 40 mg/kg, 80 mg/kg of Parmelia tinctorum preparations before irradiation respectively, then irradiated by 9 Gy 60Co γ- ray. The 30 - day survival rates and the protection factors were observed. By the same method, another group of mice were injected （ip） with Pannelia tinctorum preparations at 15min, 30min, 1 h, 2h, 4h before irradiation respectively, the 30 - day survival rates and the protection factors were observed. The protective effect of Parmelia tinctorum preparations from intramuscular and oral routes were compared with the same method. The protective effect of Parmelia tinctorum water extract preparations and alcohol extract preparations were compared. Results A certain dosage （20 - 80 mg/kg） of Parmelia tinctorum preparations could greatly improve the 30 - day survival rates and the protection factors of radiation - injured mice. Parmelia tinetorum preparations had significant therapeutic effect on 60Co γ - ray radiation - injured mice at 15min - 1 h before irradiation. Administration of Parmelia tinctorum preparations by intramuscular injection showed a little lower efficacy than by intraperitoneal injection, but administration the same dose orally had no significant radiation protective effect. Parmelia tinctorum preparations extracted by water injected （ip） before irradiation had the same radiation protective effect with those extracted by 50% alcohol. Conclusion Parmelia tinctorum preparations have a certain protective effect on acute radiation - injured mice. The best therapeutic project is injected （ip） with 40mg/kg of Parmelia tinctorum preparations at 30min before irradiation.
出处
《中国辐射卫生》
2009年第2期144-146,共3页
Chinese Journal of Radiological Health
基金
中国医学科学院放射医学研究所发展基金(SF0717)资助
关键词
石花制剂
小鼠
辐射
活存率
保护系数
Parmelia Tinctorum Preparations
Mice
Irradiation
Survival Rate
Protection Factor
