摘要
从福州动物园采集梅花鹿粪便,采用醋酸钠-抗生素分离法分离到1株苏云金杆菌(Bacillus thuringiensis,简称Bt)MHL0702,为了解该新菌株的生物学特性,对其进行了形态学、生理生化指标研究;通过PCR-RFLP鉴定体系对其cry基因型进行分析。结果表明:该菌株含有cry1Aa、cry1Ba、cry1Ea和cry1Ia类基因。对3日龄小菜蛾幼虫的室内毒力测定结果显示,该菌株对重要的农业害虫小菜蛾有较高的杀虫毒力。
The strain Bt MHL0702 was isolated using NaAc and antibiotics from the feces of a Sika deer in Fuzhou Zoo, Fujian, China. The morphology and biochemical reactions of this strain were conducted, and its cry-type genes had been identified using PCR-RFLP system. The evidence showed that this isolate harbored crylAa, crylBa, crylEa and crylIa genes. The toxicity determination in laboratory showed MHL0702 was high toxicity against the agricultural pest Plutella xylostella.
出处
《经济动物学报》
CAS
2009年第2期72-76,共5页
Journal of Economic Animal
基金
国家863课题(2006AA10A212)
福建省科技厅重点项目(2008N0050)
福建农业职业技术学院重点课题(08-EM-01)
福建省大学生科技创新项目
