摘要
有关蛋白质染色测定的分光光度研究已有不少报道，其中有些已用于蛋白质的分析测定［１～４］．锌试剂常用于金属离子的分光光度法测定，但作为生物大分子分析试剂的研究，目前还未见报道．实验表明，在ｐＨ４左右的缓冲溶液中，锌试剂与ＢＳＡ反应生成有色复合物，吸收光...
When BSA was added into zincon solution at pH 4.1, the purple colour was observed, which indicates the formation of zincon BSA complex. The maximum absorption of complex was 564 nm and 594 nm with the apparent molar absorptivity of 1 5×10 6 L·mol -1 · cm -1 . The linear range of standard curve for BSA is 0￣10 μg/mL, and the detection limit is 0 5 μg/mL. The basic reaction conditions were investigated. This proposed method was simple, sensitive, and reproducible. The analytical results of human serum albumin using this method is very satisfactory .
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1998年第6期879-881,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
