摘要
本实验对敌枯双致SD大鼠神经管畸形及其发生机制进行了研究。体重200～270g未经产雌性SD大鼠合笼交配后,于第10天上午9～10时灌服1％敌枯双-花生油混悬液(10mg/kg体重),对照组灌服等量花生油。结果表明,神经管畸形发生率为52.9％,主要是脑膨出和脑膜膨出。神经管畸形发生的形态学研究结果显示,实验组部分神经上皮细胞变性坏死;线粒体肿胀,嵴模糊甚至消失;细胞游离面出现一些大的球形突起;有的细胞逸出至管腔;细胞分裂指数明显降低;中胚层细胞空泡变性,分布稀疏,数量减少,细胞移动减慢。
30 sexually mature, virgin female SD rats, weighed 200-270 g were mated and used for the study of the teratogenic effect of N, N-methylene-bis (2-amino-1,3,4-thiadiazole) (Bis-A-TDA) on fetal neural tube formation and to explore the possible morphological mechanism of neural tube defects (NTD). In the morning of day 10 of gestation, the experimental group was administered with 10mg/kg body weight Bis-A-TDA mixed in peanut oil, and the control group with the same amount of peanut oil only. The results Showed that the incidence of NTD was 52.9% and the majority of NTD were excencephaly and encephalocele in the experimental group. In the early stage of NTD formation, some neuroepithelial cells showed vacuolated degeneration and necrosis, and the mitochondria became swollen and with indistinct or even disappeared crista. The intercellular spaces widened, and some cells escaped into the lumen of neural tube. The mitotic index of neuroepitbelial cells were sharply decreased. In the closure region of the telencephalon, similar changes of the neuroepithelium were present also, and decreased migration of mesodermal cells was noted. We consider the failure of cranial neural folds to approximate and closure was caused mainly by the damage of neuroepithelial cells, inhibition of cell proliferation, alteration of intercellular junctions and the changes of topographical arrangement of the neuroepithelium. The damage and delayed migration of mesodermal cells might also be involved in this event.
出处
《解剖学报》
CAS
CSCD
北大核心
1990年第2期215-219,共5页
Acta Anatomica Sinica
基金
国家自然科学基金
关键词
神经管畸形
形态发生
敌枯双
Neural tube defects
Morphogenesis
Ultrastructural changes
N, N'-methylene-bis (2-amino-1,3,4-thiadiazole)
Rats
