摘要
本实验比较分析了用程序控制冷冻仪进行的温度控制冷冻法和简单的液氮蒸汽快速冷冻法,对家兔桑椹胚活力的影响。快速复温后,温度控制冷冻组的胚胎体外发育率为70.27％,液氮蒸汽冷冻组中,用高浓度甘油作保护液时,胚胎体外发育串为80.56％。同步化胚胎移植后,两组的产仔率分别是13.64％和13.33％,与未经冷冻处理的对照组相似(11.11％)。结果表明,两种冷冻方法的效果相似,但液氮蒸汽冻存法比温度控制冻存法迅速,简便易行。
This paper reports the effects of the temperature control freezing and the liquid nitrogen vapour freezing on the viability of rabbit morulae. After rapid thawing, 70.27% embryos were able to develope well in vitro in the temperature control freezing group and in the liquid nitrogen vapour freezing group, the developmental rate was 80.56% when the freezing medium contained higher concentration of glycerol. After synchronous embryos were transplanted, the birth rate was 13.64% and 13.33%, respectively, in both freezing groups, and 11.11% in the control group. The results indicate that both freezing methods have almost the same good results, but the method of liquid nitrogen vapour freezing is more useful than the temperature control freezing method in some aspects.
出处
《解剖学报》
CAS
CSCD
北大核心
1990年第2期220-224,共5页
Acta Anatomica Sinica
关键词
胚胎移植
冻存方法
最佳条件
Rapid freezing
Rabbit morula
Cryoprotectant
Embryo transfer
Optimum condition
