摘要
本实验采用分离细胞体外培养和MTT微量比色法,观察了新生大鼠视网膜(R)、顶盖浅灰质(T)、间脑后外侧部(G)和枕叶皮质(C)等细胞体外培养生长的相互影响。培养3天后,MTT微量比色法测定发现:分别取自视觉系统两个部分的混合细胞培养,除G+C和T+G生长不活跃外,其余的混合培养均比单独培养的细胞生长明显活跃(高1.3～3倍不等)。其中R+T和R+G生长最为活跃,其次为R+C和T+C。另外T的条件培养液(Tc)可使R细胞生长活跃3倍,使G细胞生长活跃2倍。R的条件培养液(Rc)增强G和C细胞生长活性分别为1.3倍和2倍。以上的实验表明,T和R的条件培养液中,可能有神经诱向因子存在。
We have investigated the growth and interactions between dissociated cells from different visual structures obtained from neonatal albino rats, namely, retina (R), superficial gray of the tectum (T), the lateral posterior portion of the dieneephalon (G), and occipital cortex (C). Dissociated cells obtained were either cultured separately or mixed with another cell type in 24-well plates coated with laminin. After 3 days of culture surviving cells were quantified by the MTT colorimetric microassay.
The optical densities obtained from mixed cultures with cells from any two of the four types (G, R, T, C) of brain tissues were 1.3 to 3 times higher than the sum of their corresponding individual cultures except for those of G+C and T+G. Among them R+T and R+G were the most active cultures, followed by R+C and T+C. Further, condition medium from tectal cells (Tc) increased the activities of R and G for 3 and 2 times, respectively, and condition medium from retinal cells (Re) increased the activities of G and C for 1.3 and 2 times, respectively. Therefore it is suggested that neuronotrophic factors may be present in Tc and Rc.
出处
《解剖学报》
CAS
CSCD
北大核心
1990年第2期184-187,共4页
Acta Anatomica Sinica
基金
京港学术交流中心
Croucher基金
香港大学
永隆银行和医学研究等研究基金~~
关键词
视网膜细胞
体外培养
神经诱向因子
Retinal cells
Tectum
Diencephalon
Visual cortex
Cell culture
Neuronotrophic factor
MTT colorimetrie microassay
