摘要
采集污染的小清河污灌水,用XAD-2树脂(艹翠)酸,进行Ames试验。测试菌株用TA_(98),TA_(100),TA_(1535)和TA_(1537)平板掺入法在不加S9混合物时,相当于5升/皿的水样,上述四种菌株均呈现阳性结果。当加入S9混合物活化后,增强了对各菌株的回复突变活性,特别表现在TA_(98),呈明显剂量一回变反应关系。同时,采污灌区儿童末梢血,进行淋巴细胞微核测定。结果发现污灌区儿童微核率为0.403‰,清灌对照区儿童则为0.094‰,二者有高度显著差异。用模糊数学法分析了污灌水致突变作用与微核率的关系,贴近度((?),(?))=0.66。说明二者密切相关。污灌区儿童淋巴细胞微核率增高,可能是受污灌水中致突物质的影响。
In this paper, the irrigating sewage collected from Xioqing river were extracted with XAD-2, and their mutagenic potentials were evaluated by the salmonella reversion assay. The Ames test was performed with permeating method. When the S9 mixture was not added, the results showed that 5L/plate was mutagenic on tester strain TA98, TA100, TA1535 and TA1537. The observed activity was enhanced by the presence of Aroclor induced rat liver microsomal enzymes in all of the four tester strains. There was a doseresponse relationship between the testing doses and the increases of number of the reversional mutation strains in TA98.The test of lymphocyte micronuclei in terminal blood were performed in adolescents who lived near the place irrigated with sewage. The results showed that micronucleus rate was 0.403‰, and that i:n adolescents in control area was 0.094‰. The difference between the two places was highly significant. The Fuzzy sets analytical method was used to evaluate the impact of the mutagenic agents on the adolescents, as shown in this study, (A,B)=0.66. The experimental data indicated that the mutagenicity of irrigating sewage and lymphocyte micronucleus rates in adolescents are closely related.
出处
《解放军预防医学杂志》
CAS
北大核心
1990年第3期216-221,共6页
Journal of Preventive Medicine of Chinese People's Liberation Army
关键词
污水
突变性
淋巴细胞微核
mutagenicity, sewage, lymphocyte micronucleus, Ames test, reversional mutation
