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成熟脂肪细胞中脂联素基因表达的脂肪酸应答调控 被引量：9

Response of Fatty Acids to Adiponectin Expression in Matured Adipocytes
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摘要 用不同种类、不同浓度游离脂肪酸(FFA)处理体外培养的成熟3T3-L1脂肪细胞,实时荧光定量PCR和Western-blotting分析FFA处理前后脂联素基因mRNA和蛋白质水平的表达差异。结果表明:各类脂肪酸对脂联素mRNA的表达均具有下调作用,且抑制效应呈时间、剂量依赖性;并且各种脂肪酸显著地影响脂联素蛋白的分泌作用。 3T3-L1 preadipocytes were cultured and differentiated into the matured adipocytes in vitro. The differentiated adipocytes were incubated with different types of free fatty acids at different concentrations. Real-time quantitative PCR and Western-blotting were used to detect the expression of adiponectin mRNA and protein, respectively. The results showed that all types of fatty acids inhibitted adiponectin mRNA expression in dose and time-dependent manner. And all the fatty acids significantly influenced the secretion of adiponectin protein.
作者 袁继红 龙欢 雷霆 陈小冬 杨在清
机构地区 华中农业大学生命科学技术学院
出处 《华中农业大学学报》 CAS CSCD 北大核心 2009年第3期320-325,共6页 Journal of Huazhong Agricultural University
基金 国家"973"项目(2006CB102100) 国家"863"项目(2008AA10Z134) 国家自然科学基金项目(30771585)资助
关键词 脂肪酸 脂联素 基因表达 脂肪细胞 fatty acids adiponectin gene expression adipocytes
分类号 Q786 [生物学—分子生物学]
  • 相关文献

参考文献16

  • 1MAEDA K, OKUBO K, SHIMOMURA I, et al. cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (adipose most abundant gene transcript 1)[J]. Biochem Biophys Res Commun, 1996,221:286-289.
  • 2ARITA Y,KIHARA S, OUCHI N, et al. Paradoxical decrease of an adipocyte-specific protein,adiponectin in obesity[J]. Biochem Biophys Res Commun, 1999,257 : 79-83.
  • 3HOTTA K, FUNAHASHI T, ARITA Y, et al. Plasma concentrations of a novel adipose-specific protein-adiponectin in type2 diabetic patients[J]. Arterioscler Thromb Vasc Biol, 2000,20.. 1595-1599.
  • 4WARENSJO E, SUNDSTROM J, LIND L, et al. Factor analysis of fatty acids in serum lipids as a measure of dietary fat quality in relation to the metabolic syndrome in men[J]. Am J Clin Nutr, 2006,84 : 442-448.
  • 5JOSE-MANUEL F, JOAN V, WIFREDO R, et al. Circulating adiponectin and plasma fatty acid Profile[J]. Clinical Chemistry, 2005,51 : 603-609.
  • 6LORENTE-CEBRION S, PEREZ-MATUTE P, MARTINEZ J A,et al. Effects of eicosapentaenoic aeid(EPA) on adiponectin gene expression and secretion in primary cultured rat[J]. Physiol Biochem, 2006,62(2) :61-69.
  • 7NTAMBI J M, KIM Y C. Adipocyte differentiation and gene expression[J]. J Nutr, 2000, 130(12) : 3122-3126.
  • 8张琨,李世敏,张丽君,彭光华,张声华.血管紧张素II对3T3-L1前脂肪细胞分化的影响[J].华中农业大学学报,2008,27(1):131-134. 被引量：4
  • 9RAKAZI I, MUELLER H, RITZELER O, et al. Adiponectin counteracts cytokine-and fatty acid-induced apoptosis in the pancreatic beta-cell line INS-1 [J]. Diabetologia, 2004,47 (2) : 249-258.
  • 10LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2 ^-△△CT Method[J]. Methods, 2001,25(4) :402-408.

二级参考文献17

  • 1Weyer C, Funahashi T, Tanaka S etal. Hypoadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia. J Clin Endocrinol Metab, 2001; 86(5),1930-5.
  • 2Nakano Y, Tobe T, Choi-Miura NH et al. Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma. J Biochem (Tokyo), 1996 ; 120(4) :803- 12.
  • 3Maeda K, Okubō K, Shimomura I et al. cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1(AdiPose most abundant gene transcript 1). Biochem Biophys Res Commun, 1996;221(2):286-9.
  • 4Arita Y, Kihara S, Ouchi N etal. Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity. Biochem Biophys Res Commun, 1999;257(1): 79-83.
  • 5GIRI S,RARRAN R, HAQ E,et al. AICAR inhibits adipocyte differentiation in 3T3L1 and restores metabolic alterations in diet-induced obesity mice model[J]. Nutr Metab (Lond), 2006,3:31.
  • 6BLOMBERG A, ADLER L. Roles of glycerol and glycerol-3- phosphate dehydrogenase (NAD+) in acquired osmotolerance of Saccharomyces cerevisiae [J]. J Bacteriol, 1989, 171 (2) : 1087-1092.
  • 7JANKE J, ENGELI S, GORZEI.NIAK K, et al. Mature adipocytesinhibitin vitro differentiation of human preadipocytes via angiotensin type 1 receptors[J]. Diabetes,2002,51(6):1699- 1707.
  • 8SAINR-MARC P, KOZAK L P, AILHAUD G, et al. Angiotensin Ⅱ as atrophic factor of white adipose tissue: stimulation of adipose cell formation[J]. Endocrinology, 2001, 142 (1) : 487-492.
  • 9JONES B H, STANDRIDGE M K, MOUSTAID N. Angiotensin Ⅱ increases lipogenesis in 3T3-L1 and human adipose cells [J]. Endocrinology, 1997,138(4) : 1512-1519.
  • 10AILHAUD G. Adipose tissue as a secretory organ: from adipogenesis to the metabolic syndrome[J]. C R Biol, 2006,329 (8):570-577, 653-655.

共引文献4

同被引文献97

  • 1黄文繁,俞慕华,戴传文,严宙宁,曾钊敏.不同脂肪酸构成比食用调和油对高脂血症患者血脂代谢的影响[J].现代预防医学,2004,31(5):668-670. 被引量：7
  • 2彭士明,陈立侨,叶金云,王友慧,侯俊利,郭慧,李二超.饵料蛋白能量比对黑鲷幼鱼生长和体成分的影响[J].中国水产科学,2005,12(4):465-470. 被引量：13
  • 3屈长青,张国华,陈粉粉,赵丽丽,杨公社.猪前体脂肪细胞的原代培养[J].农业生物技术学报,2005,13(5):649-653. 被引量：43
  • 4卢建雄,杨具田,臧荣鑫,申晓蓉,马省强.胰岛素对原代培养大鼠前体脂肪细胞增殖分化的影响[J].中国兽医科学,2006,36(11):915-919. 被引量：7
  • 5Ouchi N,Kihara S,Arita Y,et al.Adiponectin,an adipocute-derived a plasma protein,inhibits endothelia NF-κB signaling through a cAmp-dependent pathway[J].Circulation,2000,102(11):1296-1301.
  • 6Mouquet F,Cuilleret E,Susen S,et al.Metabolic syndrom and collateral vessel formation inpatients with documented occuluded coronary arteries:association with hyperglycaemia insulin-resistence adiponectin and plasminogen activator inhibitor-1[J].Eur Heart J,2009,30(7):840-849.
  • 7Matsuzawa Y,Funahashi T,Kihara S,et al.Adiponectin and metabolic syndrome[J].Arterioscler Thromb Vasc Biol,2004,24(1):29-33.
  • 8Pischon T,Rimn EB.Adiponectin and risk of acute coronary syndromes:defining the obesity phenotyper[J].Eur Heart J,2007,28(3):292-298.
  • 9Kumada M,Kihara S,Ouchi N,et al.Adiponectin specifically increased tissue inhibitor of metalloproteinase-1 through interleukin-10 expression in human macrophages[J].Circulation,2004,109(17):2046-2049.
  • 10Scherer PE,Williams S,Fogliano M,et al.A novel serum protein similar to C1q,produced exclusively in adipocytes[J].J Biol Chem,1995,270(45):26746-26749.

引证文献9

二级引证文献27

华中农业大学学报
2009年 第3期

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