摘要
从新生牛和新生大白鼠的肝脏中提取出了肝刺激物质(HSS)。新生牛肝HSS促进豚鼠肝细胞DNA合成作用较强(p/o:5.72),75℃,20min和100℃,1.5min处理后活性无明显改变。新生大白鼠肝HSS原液在0.05mg/ml时呈最佳使用浓度,增加和减少其浓度,生物活性都逐渐下降。活性组份以分子量约为14,000和23,000Dalton为主。对照大白鼠肝制备液无HSS活性,并且分子量约为23,000Dalton的蛋白质组份很少或没有。结果表明:新生动物如牛和大白鼠的肝脏都含有HSS,且活性较强,来源丰富,可作为HSS较理想的来源。
The HSS found in the cytosol of new-born calf liver was heat-stable and it promoted the DNA-synthesis of guinea-pig liver cells (P/o:5.72),HSS was also found in the cytosol of new-born rat livers,and its most effective concentration to promote incorporation of H2-TdR into DNA of guinea-pig liver cells was about 0.05mg/ml.This activity was reduced with both the increase and decrease of cytosol content,and the reason was not clear.It was proved with SDS-PAGE that the molecular weight of the active component was about 14000 and 23000 daltons.The cytosol from control rat livers showed no HSS activity and also no component measuring 23000 daltons.It needs further identification that the 23000 daltons protein is the active component in the cytosol of new-born calf and rat livers and the partially hepatectomized rat livers,but not in the cytosol of control rat livers.This finding indicateds that the livers of new-born calf and rat contain highly active HSS and are a valuable resource of HSS
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1990年第4期248-250,共3页
Medical Journal of Chinese People's Liberation Army
关键词
肝刺激物质
DNA合成
肝细胞再生
Hepatic stimulatory substance DNA-synthesis Liver cell proliferation
