摘要
目的探讨p63与CD44在皮肤衰老过程中与年龄、紫外线的关系。方法采用免疫组化方法检测121例正常全厚皮肤标本中p63与CD44的表达，光镜下观察并计算表皮基底层p63阳性细胞百分比，用图像分析软件image proplus 6．0测量表皮层p63与CD44表达的吸光度值。结果①表皮基底层p63阳性细胞百分比与年龄呈负相关（r=-0．218，P〈0．05），51～79岁组最低；但在曝光部位、半曝光部位与非曝光部位间的差异无统计学意义。②表皮p63的表达强度与年龄在曝光部位呈负相关（r=-0．389，P=0．010），在半曝光部位无相关性，在非曝光部位呈正相关（r=0．341，P〈0．05）；三个部位表达强度的差异在10～30岁组无统计学意义，在31～50岁组与51～79岁组由强至弱依次为非曝光部位、半曝光部位、曝光部位。③表皮CD44的表达强度与年龄之间呈微弱负相关（r=-0．083，P〈0．05），10～30岁组最高；但在曝光部位、半曝光部位与非曝光部位间的差异无统计学意义。结论表皮基底层p63阳性细胞百分比、表皮CD44的表达强度和年龄呈负相关，和紫外线照射量无关。表皮p63表达强度在31～50岁组与51～79岁组的表达强度依曝光多少而递减。
Objective To clarify the relationship of p63 and CD44 expressions with age and ultraviolet irradiation in the process of skin aging. Methods Full-thickness skin samples were obtained from the normal skin at different sites, including 44 sun-exposed sites （face, neck, dorsal aspect of the forearm）, 46 sun-protected sites （abdomen, thigh）, and 31 semi-exposed sites （back, flexor aspect of the forearm, upper arm, leg）, of 121 volunteers （63 males and 58 females）. Of all the volunteers, 55 were 10 to 30 years of age （young）, 41 were 31 to 50 years of age （adult）, and 25 were 51 to 79 years of age （old）. Immunohistochemisty using antibodies to p63 and CD44 was applied to detect the expression of p63 and CD44 in these specimens. Imaging analysis software was utilized to calculate the percentage of p63-expressing cells in basal layer as well as the expression level of p63 and CD44. Results In stratum basale, the percentage of p63-expressing cells decreased with age （r = -0.218, P 〈 0.05 ）, but showed no significant difference among sun-exposed skin, semi-exposed skin and sun-protected skin （P 〉 0.05）. The correlation between the expression intensity of epidermal p63 and age was negative in sun-exposed sites （r = -0.389, P = 0.010）, positive in sun-protected sites （r = 0.341, P 〈 0.05）, but non-significant in semi-exposed sites （P 〉 0.05）. With regard to the expression of epidermal p63, no significant difference was observed among sunexposed sites, sun-protected sites and senti-exposed sites in volunteers aged from 10 to 30 years, while in those aged from 31 to 50 years and from 51 to 79 years, sun-protected sites was significantly higher than semi-exposed sites followed by sun-exposed sites （all P 〈 0.05 ）. There was a weak correlation between the expression intensity of epidermal CD44 and age （r = -0.083, P 〈 0.05 ）, but no significant difference was noted among the three kinds of sites （P 〉 0.05）. Conclusions In stratum basale, the percentage of p63- expressing cells and the expression intensity of CD44 negatively correlate with age, but have no correlation with ultraviolet irradiation. In individuals aged from 31 to 50 years and from 51 to 79 years, the expression of epidermal p63 decreases with the accumulation of sun-exposure.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2009年第6期393-395,共3页
Chinese Journal of Dermatology
