摘要
目的:探讨白首乌的3个C21甾体皂苷类成分对人肺癌A549细胞生长的抑制作用及对细胞周期的影响。方法:按浓度梯度配置3个C21甾体皂苷类成分,使终浓度分别为5,10,20,40,60,80,100μmol.L-1,采用MTT法检测3个C21甾体皂苷类成分作用48 h各浓度对人肺癌A549细胞生长的抑制作用;分别选择3个C21甾体皂苷类成分的IC50近似浓度(47,34,60μmol.L-1)处理细胞12,24,48 h后,采用流式细胞仪检测对细胞周期变化的影响。结果:分离得到的3个C21甾体皂苷:隔山消苷C1N、隔山消苷C3N、隔山消苷K1N作用48 h的IC50分别为46.07,33.02,59.92μmol.L-1;细胞周期分析显示,随着作用时间的延长(12,24,48 h),隔山消苷C1N、隔山消苷C3N使G0/G1期细胞数逐渐增加,S和G2/M期细胞数逐渐减少(P<0.05,P<0.01);隔山消苷K1N使S期细胞数减少,G2/M期细胞数增加(P<0.05,P<0.01)。结论:3个甾体皂苷对A549细胞均有不同程度的生长抑制作用,并呈浓度依赖性,其作用机制可能与3个甾体皂苷诱导细胞周期阻滞有关。
Objective： To investigate the inhibition of three Cn steroidal saponins from Cynanchum auriculatum on the cell growth and cell cycle of human lung cancer A549 cells. Method ： A549 cells were exposed to three C21 steroidal saponins of different concentrations （5, 10, 20, 40, 60, 80, 100 μmol·L^-1） for 48 hours. After 48 h, MTT assay was used to evaluate the inhibiting effects of three C21 steroidal saponins on the proliferation of the A549 cells. Exponential growth phase A549 cells were treated with 47, 34, 60 μmol· L^-1 of three C21 steroidal saponins respectively for 12, 24, 48 h, then the changes of cell cycle were analyzed by flow cytometry. Result： The three C21 steroidal saponins could inhibit the growth of A549 cells in dose-dependent manner and IC50 is 46.07mol·L^-1, 33.02 mol·L^-1, 59.92 mol·L^-1 respectively. The cell cycle analysis showed that wilfoside C1 N and wilfoside C3 N increased the percentage of G0/G1 phase cells and decreased G2/M and S-phase cells while the proportion of cells in S-phase was lower and in G2/M phase the proportion was higher than control when the cells were cultivated with wilfoside K1N （P 〈 0. 05, P 〈 0. 01 ）. Conclusion： Three C21 steroidal saponins could inhibit the proliferation of A549 ceils in dose-dependent manner and the mechanism may be related to its arresting the cell cycle.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第11期1418-1421,共4页
China Journal of Chinese Materia Medica
基金
江苏省科技厅公益项目(BM2004525)
关键词
白首乌
C21甾体皂苷
生长抑制
细胞周期
Cynanchum auriculatum
C21 steroidal saponins
growth inhibition
cell cycle
