摘要
目的血管紧张素原是血管活性多肽－血管紧张素Ⅱ的唯一前体，又是急期蛋白之一。因此，研究ＩＬ－６调节血管紧张素原基因表达对阐明人体感染时血浆血管紧张素Ⅱ水平增加致高血压有重要意义。方法应用Ｎｏｒｔｈｅｒｎ印迹杂交技术检测了ＩＬ－６刺激的肝癌细胞Ｈｅｐ３Ｂ中血管紧张素原ｍＲＮＡ，并且进一步通过ＤＮＡ－蛋白质电泳泳动漂移、ＤＮＡ重组和瞬时转染技术分析了人血管紧张素原基因５′端侧翼序列－５６８位置的一个ＩＬ－６反应元件同源序列（ＨＡＧＩＬ－６ＲＥ）。结果ＩＬ－６使血管紧张素原ｍＲＮＡ明显提高，位于血管紧张素原基因启动子中的ＨＡＧＩＬ－６ＲＥ结合于转录因子ＣＡＡＴ／增强子结合蛋白（Ｃ／ＥＢＰ）；将多拷贝的ＨＡＧＩＬ－６同ＴＫ核心启动子连接，再与氯霉素乙酰转移酶（ＣＡＴ）基因融合，构成异源表达载体，转染ＨｅｐＧ２细胞，发现ＩＬ－６增强Ｃ／ＥＢＰα的作用活性。结论Ｃ／ＥＢＰ在ＩＬ－６诱导血管紧张素原基因表达中具有调节作用。
Objective Angiotensinogen is the only known precursor of vasoactive angiotensinⅡ and also one of the acute phase proteins. This study was intended to understand the regulation of angiotensinogen gene expression induced by IL 6. Methods Northern hybridization, electrophoretic mobility shift assay and transient transfections were conducted. Results Northern hybridization showed increase of angiotensinogen mRNA treated by IL 6 in Hep3B cells. Electrophoretic mobility shift assay further indicated the HAG IL 6RE homologous to IL 6 responsive element at -568 site of the angiotensinogen promoter binds C/EBP(CAAT/Enhancer Binding Protein).Consistent with this binding studies were the transient transfections of the expression vector in which 6 copies of HAG IL 6RE linked to TK core promoter and fused to CAT reporter gene, revealing that C/EBPα was a transactivator under IL 6 induced condition. Conclusion These observations suggest that C/EBP plays regulatory role in IL 6 induced angiotensinogen gene expression.
出处
《中华医学遗传学杂志》
EI
CAS
CSCD
北大核心
1998年第3期129-132,共4页
Chinese Journal of Medical Genetics
基金
国家自然科学基金
