摘要
目的研究仙灵骨葆对卵巢切除大鼠股骨骨折愈合的作用。方法健康12周龄雌性SD大鼠40只,体重(258±14)g,随机分为4组,每组10只。A组仅作切口暴露腹腔;B组仅切除卵巢;C、D组切除卵巢同时制备右侧股骨中段横型骨折,髓内针固定,术后分别采用生理盐水及250mg/(kg·d)仙灵骨葆灌胃。于术后即刻、1、2、3、4及5周测量A、B组大鼠体重。术后5周取各组右侧股骨标本,双能X线骨密度仪测量股骨全长骨密度(total femur bone mineral density,tBMD)、远1/3段骨密度(distal femur bone mineral density,dBMD)和中1/3段骨密度(middle femur bone mineral density,mBMD),并行C、D组CR摄片、HE染色和免疫组织化学染色。结果B组大鼠体重从第3周开始显著高于A组(P<0.05),绝经后骨质疏松模型制备成功。CR摄片示D组骨痂量相对较多,骨折线模糊;C组骨痂量较少,骨折线清晰。B组tBMD和dBMD明显低于A组,D组mBMD明显高于C组,差异均有统计学意义(P<0.05);D组tBMD和dBMD较C组有增高趋势,但差异无统计学意义(P>0.05)。组织学观察D组与C组比较,骨折端大部分骨性愈合,骨髓腔可见较多毛细血管植入。免疫组织化学染色示C、D组BMP-2的积分吸光度(IA)值分别为2.2366±0.1818和3.7273±0.8742,VEGF的IA值分别为2.8355±0.5370和3.8396±0.2230,差异均有统计学意义(P<0.05)。结论仙灵骨葆可促进卵巢切除大鼠股骨骨折愈合,加快编织骨向板层骨的转化,这可能与上调BMP-2和VEGF表达相关。
Objective To evaluate the effects of XiangLingGuBao （XLGB） on femoral fracture healing in ovariectomized （OVX） rats. Methods Forty 12-week-old female SD rats weighing （258±14）g were divided randomly into 4 groups （n=10 per group）： group A, sham operation by opening the abdominal cavity; group B, bilateral ovariectomy; group C, bilateral ovariectomy, transverse midshaft fracture of the right femur with intramedullary nail fixation, and normal saline by gavage; group D, bilateral ovariectomy, transverse midshaft fracture of the right femur with intramedullary nail fixation, and 250 mg/（kg·d） XLGB by gavage. The weight of rabbits in groups A and B was measured 0, 1, 2, 3, 4 and 5 weeks after operation. The right femur of each rat was obtained 5 weeks after operation. Total femur bone mineral density （tBMD）, distal femur bone mineral density （dBMD） and middle femur bone mineral density （mBMD） were measured by double energy X-ray absorptiometry CR filming, HE staining and immunohistochemistry staining of groups C and D were performed. Results The weight of rats in group B was obviously higher than that of group A at 3, 4 and 5 weeks after operation （P 〈 0.05）, indicating the animal model of postmenopausal osteoporosis was established successfully. CR films showed more callus and obscure fracture 1 ine in group D, while less callus and distinct fracture 1 ine in group C. The tBMD and the dBMD of group B were far less than that of group A, the mBMD of group D was significantly higher than that of group C （P 〈0.05）, the tBMD and the dBMD of group D were higher than that of group C, but no significant difference was evident between two groups （P 〉 0.05）. Histology observation showed, when compared with group C, most fracture ends in group D reached bone union, and the introduction of capillaries was evident in the marrow cavity. Immunohistochemistry staining demonstrated that the BMP-2 integrated absorbance （IA） value in groups C and D was 2.2366±0.1818 and 3.7273±0.8742, respectively, the VEGF IA value in groups C and D was 2.8355±0.5370 and 3.8396±0.2230, respectively, indicating there were significant differences between two groups （P 〈0.05）. Conclusion XLGB can obviously promote the femoral fracture healing in OVX rats, and speed the transformation of woven bone into lamellar bone, which may rely on its role of enhancing expression of BMP-2 and VEGF.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2009年第6期690-693,共4页
Chinese Journal of Reparative and Reconstructive Surgery
