摘要
目的:研究Cortactin对大肠癌细胞运动性和侵袭力的影响.方法:HT-29细胞常规培养,在体外合成Cortactin siRNA后转染结肠癌细胞,通过Western blot和免疫荧光观察转染后Cortactin表达量变化,通过癌细胞体外迁移侵袭实验和伸展实验来观察转染后癌细胞伸展、迁移和侵袭能力的改变.结果:Western blot和免疫荧光显示Cortactin在siRNA处理后癌细胞内的表达水平明显下降;癌细胞经过Cortactins iRNA处理后细胞的胞膜展开比对照组晚;Cortactin siRNA处理的大肠癌细胞和对照GFPsiRNA处理的癌细胞通过涂胶基膜和微孔的细胞数目统计分别为60±15个和340±45个,两者差异有统计学意义(P<0.01).结论:Cortactin可能对大肠癌的侵袭转移发挥作用.
AIM： To examine the role of Cortactin in motility and invasive ability of human colon cancer cells.
METHODS： HT-29 colon cancer cells were cultured and treated with Cortactin siRNA. After treatment, the expression level of Cortactin was detected by western blot assay and the expression in cells was assayed under confocal. For test, cells were seeded in 6-well plates and the spreading of cancers was observed. Furthermore, cells were seeded into chambers coated with collagen, and the migration and invasiveness were examined by counting the cells pass
ing through the mini pores on chamber.
RESULTS： Cortactin in cancer cells were knocked down dramatically. The cancer cells treated with Cortactin siRNA demonstrated a postponed spreading, less migration and attenuated invasive ability, compared with cancer cells treated with GFP siRNA. After Cortactin RNAi, the cells that passed through mini-pores was 60 ± 15, while it was 340 ± 45 in the control group （P 〈 0.01）.
CONCLUSION： Cortactin might play an important role in invasion and metastasis of colon cancer.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第12期1197-1201,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30771126
No.30772106
江苏省自然科学基金资助项目
No.BK2006058~~
