摘要
建立了同时监测发酵液中香兰素、香兰素酸、香兰素醇和阿魏酸的高效液相色谱法(HPLC)。色谱柱为Eclipse Zorbax XDB-C18(4·6mm×150mm,5μm),流动相为甲醇∶水∶冰醋酸(35∶65∶0·05),流速为0·80mL/min,检测波长为260nm和280nm。在此色谱条件下,四种物质获得良好的分离,且在一定浓度范围内,各组分的线性关系、方法的精密度和回收率都令人满意。
This paper set up a method to monitor van fermentation broth simultaneously by high performance n, vanillin acid, vanillin alcohol and ferulic acid in the liquid chromatography （HPLC） .The chromatography column used was an Eclipse Zorbax XDB-C18 column （4.6mm × 150mm, 5μm）, and the mobile phase was methanol：water：acetic acid（35：65：0.05）.The flow rate was 0.80mL/min and the detection wavelengths were 260nm and 280nm.Under this selected chromatography conditions, the four substances could be separated effectively.Meanwhile, within certain concentration range ,the components had good linear relation and the method had good precision, as well as satisfactory recovery.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第5期332-333,345,共3页
Science and Technology of Food Industry
