摘要
目的：探讨表皮生长因子受体（ＥＧＦＲ）基因表达与胶质瘤恶性程度的关系。方法：采用Ｎｏｒｔｈｅｒｎ印迹杂交及免疫组织化学技术从ｍＲＮＡ及蛋白水平检测了５０例人脑胶质瘤、４个体外恶性胶质瘤细胞系及８例正常脑组织的ＥＧＦＲ的基因表达。结果：免疫组化检测高恶度胶质瘤（ＷＨＯⅢ－Ⅳ级）ＥＧＦＲ表达６９％（２０／２９），而低恶度胶质瘤（ＷＨＯⅠ－Ⅱ级）表达率为３３％（７／２１），差异显著（Ｐ＜０．０１）；４例细胞系阳性表达１００％；８例正常脑组织无ＥＧＦＲ蛋白表达。ＷＨＯ分级与ＥＧＦＲ蛋白表达呈正相关（ｒ＝０．５５９７，Ｐ＜０．００１）。对５０例胶质瘤进行Ｎｏｒｔｈｅｒｎ印迹杂交，其结果与免疫组化检测结果一致，ＥＧＦＲｍＲＮＡ也随胶质瘤恶性程度的增加而表达增高。结论：ＥＧＦＲ可能在恶性胶质瘤的发生发展中起重要作用，ＥＧＦＲ过表达与胶质瘤分级相关性为从分子水平评估肿瘤的恶性程度及选择基因治疗的靶基因提供了参考。
Objective: To investigate the expression of EGFR gene in human gliomas. Methods: 50 human gliomas, 4 malignant human glioma cell lines and 8 normal brain tissues were studied by immunohistochemical and Northern blot analysis. Results: It was found by immunohistochemical study that highgrade gliomas (WHO ⅢⅣ grade) expressed positive staining cell in 69%, whereas low grade gliomas (WHO ⅠⅡ grade) in 333% of cases (P<001), all 4 malignant human cell lines expressed EGFR, while 8 normal brain samples had no EGFR protein expression. The EGFR expression was significantly increased in ascending order from grade I to grade IV gliomas (r=05597, P<001). The results of Northern blot analysis on 50 samples of gliomas were concidente with immunohistochemical staining, and Northern blot analysis also indicated that overexpression of EGFR mRNA was correlated with the degree of malignancy of gliomas. Conclusion: EGFR gene may play an important role in the malignant progression in gliomas. The overexpression of EGFR gene aslo provide an useful parameter in evaluating the degree of malignancy at molecular level and selecting the target of gene for gene therapy.
出处
《中华神经外科杂志》
CSCD
北大核心
1998年第2期71-74,共4页
Chinese Journal of Neurosurgery
基金
国家自然科学基金
