摘要
目的确定ＭＲＳＡ耐药基因的来源及传播途径，为ＭＲＳＡ流行病学分析提供重要参考。方法对实验菌株做药敏实验、ＭＲＳＡ鉴定、ＭＲＳＡ质粒提取、质粒转化和消除实验，并将提取的多耐药质粒（２３Ｋｂ）进行酶切，应用重组克隆技术，将各酶切片段转化到非耐药受体菌Ｅ．ｃｏｌｉＪＭ１０９中，筛选和确认重组子，经药敏实验分析ＭＲＳＡ耐药基因的位置、大小以及与多重耐药性的关系。结果本实验菌株的耐药谱、质粒外型及酶切图谱极相似，提示有暴发感染的可能性。结论该Ｒ－质粒含有多个３．０Ｋｂ左右的重复耐药基因，包含耐药基因ＤＮＡ片段所诱导的耐药程度。
Objective This study may supply an important evidence for epidemiological study of hospital infection. in our hospital. Methods The strains were subjected to drug susceptibility tests, MRSA strains plasmid extract, transferred and eliminated test. The multidurg resisant plasmid (23Kb) was digested. By recombinant DNA technique, the four DNA fragments were transferred into E. coli JM109, that screen out the bacteria containing DR gene. The drug susceptibility tests were used to analyse the location and size. Results The strains used as subject of test shared similar DR picture, plasmid pattern and DNA restriction profiles. Conclusion The 23Kb plasmid contained 3. Okb reiterated DR gene. The strains transferred with DNA fragment of DR genes showed weaker resistant to drugs than the strains transferred with the whole DR plasmid.
出处
《中华医院感染学杂志》
CAS
CSCD
1998年第2期72-74,共3页
Chinese Journal of Nosocomiology
关键词
MRSA
耐药性
质粒图谱
基因定位
MRSA Drug resistant (DR) Plasmid mapping Gene location
