摘要
目的改进红细胞渗透脆性测定方法。方法545nm波长下分别测定红细胞在低渗氯化钠溶液、加维生素C的低渗氯化钠溶液和加桔梗煎剂的低渗氯化钠溶液中溶血后的光密度。结果红细胞在低渗氯化钠溶液中溶血后的溶血率与传统肉眼观察结果一致,而红细胞在加维生素C的低渗氯化钠溶液和加桔梗煎剂的低渗氯化钠溶液中的溶血率与红细胞在正常低渗氯化钠溶液的溶血率具有显著差异。结论采用红细胞溶血后的光密度作为检测指标,结果符合红细胞渗透脆性生理特性,也可作为临床评价药物对红细胞渗透脆性影响的指标。
Objective To improve a method for determining erythrocyte osmotic fragility. Methods OD values at 545 nm were measured after the hemolysis of red blood cells in solution of hypotonic sodium chloride, solution of hypotonic sodium chloride with vitamin C, and solution of hypotonic sodium chloride with Campanulaceae decoction, respectively. Results The hemolytic rates in solution of hypotonic sodium chloride were consistent with that detected by traditional visual observation. The hemolytic rates in solution of hypotonic sodium chloride with vitamin C or solution of hypotonic sodium chloride with Campanulaceae decoction were significantly different from those in normal solution of hypotonic sodium chloride. Conclusion The optical density after hemolysis of red blood cell is in line with the physical properties of osmotic fragility,and can be used as a indicator to evaluate the osmotic fragility of red blood cell after drug treatment in clinic.
出处
《山西医科大学学报》
CAS
2009年第5期391-393,共3页
Journal of Shanxi Medical University
基金
湖北省郧阳医学院教学研究基金资助项目(20060170025)
关键词
分光光度法
红细胞渗透脆性
溶血率
spectrophotometric method
erythrocyte osmotic fragility
hemolytic rate
