摘要
背景:鼠伤寒沙门氏菌回复突变试验具有一定的预测致癌物的能力,其敏感性、特异性、准确性较高。目的:通过对聚β-羟基丁酸酯材料进行鼠伤寒沙门氏菌回复突变试验,评价该材料的潜在致突变性。设计、时间及地点:体外对比观察实验,于2009-02/03在国家沈阳新药安全评价研究中心实验室完成。材料:聚β-羟基丁酸酯(10mm×10mm)由中国科学院金属研究所提供,组氨酸营养缺陷型鼠伤寒沙门氏菌TA97,TA98,TA100,TA102由沈阳市安全评价中心遗传毒性室提供。方法:采用标准平板掺入法,计数TA97,TA98,TA100,TA102标准测试菌株在4个不同浓度浸提下,计算于37℃培养48h后的回复突变菌落数。加S9混合液作为体外代谢活化系统。主要观察指标:各平皿细菌回复突变菌落数,凡高于阴性对照2倍以上者即为阳性结果。结果:聚β-羟基丁酸酯细菌回复突变试验显示,各剂量组细菌回复突变数小于阴性对照组细菌回复突变数的2倍。各菌株对应各剂量组无论代谢活化与否,皆为阴性试验结果。结论:聚β-羟基丁酸酯经细菌回复突变试验未见潜在致突变性。
BACKGROUND： Salmonella typhimurium Ames test has the ability to predicting carcinogen and high sensitivity, specificity, and accuracy.
OBJECTIVE： To evaluate the potential mutagenicity of poly-beta-hydroxybutyrate （PHB） via salmonella typhimurium Ames test. DESIGN, TIME AND SETTING： An in vitro contrast study was performed at the laboratory of Shenyang Xinyao Safety Evaluation Center from February to March 2009.
MATERIALS： PHB sized 10 mm × 10 mm was provided by Metal Institute of Chinese Academy of Science; histidine-auxotroph spontaneous revertants TA97, TA98, TA100, and TA102 were provided by Genetoxic Laboratory of Shenyang Safety Evaluation Center. METHODS： In the fiat-plate incorporation test of salmonella typhimurium, the average number of spontaneous revertants of TA97, TA98, TAI00, and TA102 at four concentrations was calculated after incubation at 37 ℃ for 48 hours. S9 mixed liquid was used as metabolism activation system in vitro.
MAIN OUTCOME MEASURES： The number of spontaneous revertants which was twice more than negative controls was considered as the positive one. RESULTS： Salmonella typhimurium Ames test demonstrated that the number of spontaneous revertants was twice less than negative control group. The result of Ames test for PHB was negative.
CONCLUSION： PHB has no potential mutagenicity via salmonella typhimurium Ames test.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第21期4137-4140,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
辽宁省教育厅2005年度高等学校科研项目(05L481)~~
