摘要
从犬粪便中分离到1株细小病毒,在F81细胞出现明显的CPV病变,电镜观察可见细小病毒样粒子,进一步理化性质鉴定该病毒具有细小病毒的特征,表明所分离病毒为CPV,命名为CPV-04-08-CN-6。根据特异性引物,采用PCR技术扩增出VP2全长基因,将PCR产物克隆入pMDl8-T载体,进行测序分析。结果,CPV04-08-CN-6VP2基因全长1755bp,编码584个氨基酸,与CPV参照株的VP2基因核苷酸同源性为99.69%,12个核苷酸发生错义突变,导致4个氨基酸发生变异。VP2基因的系统发生分析表明CPV-04/08/CN-6在细小病毒分类上属于CPV-2a型。
A canine parvovirus strain, isolated from the feces of dog in animal hospital of Animal Science and Technology Institule, Sichuan Agricultural University, produced specific cytopathic effects in F81 cells, By morphological identification, electron microscopy shows that small virus-like particles, and further identification of physical and chemical properties, found that the virus has the characteristics of parvovirus, indicating the virus was isolated by the CPV, named CPV-04-08-CN-6. According to the specific primer, using PCR technology to amplify the full-length VP2 gene, PCR product was cloned into pMD 18-T vector for sequencing. The results, CPV-04-08-CN-6 VP2 gene length 1755bp, encoding 584 amino acids, and the strain of CPV in the light of the standard VP2 gene nucleotide homology of 99.69%, Amino acid homology was 99.58%,12-nucleotide mutation occurred, resulting in 4 Mutate amino acids. VP2 gene analysis showed that CPV-04/08/CN-6 in the parvovirus classification belongs to CPV-2a.
出处
《现代生物医学进展》
CAS
2009年第10期1888-1890,1894,共4页
Progress in Modern Biomedicine
基金
国家林业局野生动物疫源疫病本底调查
关键词
CPV
VP2基因
序列分析
Canine Parvovirus
VP2 gene
Sequence analysis
