摘要
目的观察胚胎运动神经元种植对在体失神经骨骼肌萎缩的影响。方法取孕12～14天的胎鼠运动神经元种植至成鼠失神经腓肠肌内,于实验后9、22周取双侧腓肠肌并测定肌湿重;然后行ATP酶组织化学及肌动蛋白免疫组织化学染色,并作图像分析。结果实验组腓肠肌的肌湿重、Ⅰ和Ⅱ型纤维面积、肌动蛋白的相对含量均明显优于对照组(P<0.05)。结论胚胎运动神经元种植至失神经骨骼肌内是一种防治失神经肌萎缩的有效方法。
Objective To observe the effects of transplanted embryonic motoneurons on denervated skeletal muscle atrophy in vivo. Methods The adult rats in experimental group received the implantation of embryonic motoneurons of the rats with the age of 12—14 days old to the denervated gastrocnemius muscles. Bilateral gastrocnemius muscles of each rat from the two groups were taken and weighed at 9th and 22nd post-operative weeks, repsectively. The above muscles underwent ATP ase histochemical and antiactin immunohistochemical staining, and then image analysis. Results The muscle wet weight and the cross-sectional areas of type Ⅰ and Ⅰ fibers, and the actin content of the rats in the experimental group were significantly increased as compared with in the control group (P<0.05). Conclusion The implantation of embryonic motoneurons into denervated muscles may be a long-term effective method to retard the progress of muscle atrophy.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1998年第4期364-365,共2页
Chinese Journal of Experimental Surgery
基金
美国中华医学会基金(No.95619)
关键词
胚胎
运动神经元
肌萎缩
肌神经移植
Embryo
Motoneurons
Denervated skeletal muscle
Allograft
Atrophy
